Variation of the group 5 grass pollen allergen content of airborne pollen in relation to geographic location and time in season - 03/07/15
, Marje Prank, MSc c, Mikhail Sofiev, PhD c, Gudrun Pusch, Dipl Ing a, b, Roberto Albertini, PhD d, Isabella Annesi-Maesano, MD, DSc, PhD e, f, Celia Antunes, PhD g, h, Heidrun Behrendt, MD a, b, Uwe Berger, MBA i, Rui Brandao, PhD g, †, Sevcan Celenk, PhD j, Carmen Galan, PhD k, Łukasz Grewling, PhD l, Bogdan Jackowiak, PhD l, Roy Kennedy, PhD m, Auli Rantio-Lehtimäki, PhD n, Gerald Reese, PhD o, Ingrida Sauliene, PhD p, Matt Smith, PhD i, Michel Thibaudon, Pharm q, Bernhard Weber, PhD o, Lorenzo Cecchi, MD rAbstract |
Background |
Allergies to grass pollen are the number one cause of outdoor hay fever. The human immune system reacts with symptoms to allergen from pollen.
Objective |
We investigated the natural variability in release of the major group 5 allergen from grass pollen across Europe.
Methods |
Airborne pollen and allergens were simultaneously collected daily with a volumetric spore trap and a high-volume cascade impactor at 10 sites across Europe for 3 consecutive years. Group 5 allergen levels were determined with a Phl p 5–specific ELISA in 2 fractions of ambient air: particulate matter of greater than 10 μm in diameter and particulate matter greater than 2.5 μm and less than 10 μm in diameter. Mediator release by ambient air was determined in FcεRI-humanized basophils. The origin of pollen was modeled and condensed to pollen potency maps.
Results |
On average, grass pollen released 2.3 pg of Phl p 5 per pollen. Allergen release per pollen (potency) varied substantially, ranging from less than 1 to 9 pg of Phl p 5 per pollen (5% to 95% percentile). The main variation was locally day to day. Average potency maps across Europe varied between years. Mediator release from basophilic granulocytes correlated better with allergen levels per cubic meter (r2 = 0.80, P < .001) than with pollen grains per cubic meter (r2 = 0.61, P < .001). In addition, pollen released different amounts of allergen in the non–pollen-bearing fraction of ambient air, depending on humidity.
Conclusion |
Across Europe, the same amount of pollen released substantially different amounts of group 5 grass pollen allergen. This variation in allergen release is in addition to variations in pollen counts. Molecular aerobiology (ie, determining allergen in ambient air) might be a valuable addition to pollen counting.
Le texte complet de cet article est disponible en PDF.Key words : Allergen, grass, Phl p 5, Europe, PM10, PM2.5, exposure, allergy, basophil, modeling, SILAM, HIALINE
Abbreviations used : HIALINE, 10>PM>2.5, PM>10
Plan
| This publication arises from HIALINE, a project that has received funding from the European Union in the framework of the Health Programme. |
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| Disclosure of potential conflict of interest: J. Buters has received or has grants pending from Foundation CK-CARE. S. Celenk's institution has received funding from the Scientific and Technological Research Council of Turkey (TUBITAK 109S032). B. Weber is employed by Allergopharma GmbH & Co. KG. The rest of the authors declare that they have no relevant conflicts of interest. |
Vol 136 - N° 1
P. 87 - juillet 2015 Retour au numéro
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