Reduced expression of TRIF in chronic HBV infected Iranian patients - 30/10/13

Doi : 10.1016/j.clinre.2012.11.005

Fatemeh Ayoobi ^a, Gholamhossein Hassanshahi ^b, Nahid Zainodini ^c, Hossein Khorramdelazad ^b, Mohammad Kazemi Arababadi ^c,^⁎ , Derek Kennedy ^d
^a Physiology and Pharmacology Research center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
^b Molecular Medicine Research center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
^c Immunology of Infectious Diseases Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
^d School of Biomolecular and Physical Science, Eskitis Institute for Cell and Molecular Therapies, Griffith University Nathan, Queensland, Australia

^⁎Corresponding author. Immunology of Infectious Diseases Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. Tel.: +00983915234003 5; +00989139922445; fax: +00983915225209.

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Summary

Background and aims

TRIF is one of the main intracellular adaptor proteins required for TLR3 and 4 signaling. Abnormal gene expression of TRIF may lead to abrogated immune responses against viral infections including hepatitis B infection. The aim of this study was to identify the mRNA levels of TRIF in PBMCs isolated from chronic HBV (CHB) infected patients.

Material and methods

mRNA was isolated from 63 CHB patients and 60 healthy controls and transcript levels of TRIF were examined in parallel with beta-actin (as housekeeping gene) using Real-Time PCR techniques.

Results

Our results demonstrated that expression of TRIF was significantly decreased in PBMCs isolated from CHB patients when compared to healthy controls.

Conclusions

Based on the results reported here, it seems that CHB patients are unable to express appropriate levels of the TRIF gene, which may attenuate TLR3 and 4 signaling subsequent to HBV infection. Our results suggest a possible mechanism, which may explain why hepatitis B infection is stable in CHB patients.

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Abbreviations : HBV, PBMC, TRIF, TLR, PAMP, MYD88, IRAK1, TRAF6, NF-κB, IRF3

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Introduction

Material and methods

Subjects

Detection of serological HBV markers

HBV-DNA extraction and Real-Time PCR conditions

RNA extraction, reverse transcription and quantitative Real-Time PCR

HBV genotyping

Liver enzymes detection

Data analysis and statistical methods

Results

Detection of HBsAg and HBeAg

Quantification of HBV-DNA copy numbers

Expression of TRIF

HBV genotyping

Detection of liver enzymes

Discussion

Disclosure of interest

Esportazione

Vol 37 - N° 5

P. 491-495 - Novembre 2013 Ritorno al numero

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Reduced expression of TRIF in chronic HBV infected Iranian patients - 30/10/13

Summary

Background and aims

Material and methods

Results

Conclusions

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