Identification and immunologic characterization of an allergen, alliin lyase, from garlic (Allium sativum) - 24/08/11
Abstract |
Background |
Garlic (Allium sativum) is one of the most common relishes used in cooking worldwide. Very few garlic allergens have been reported, and garlic allergy has been rarely studied.
Objective |
The aim of the study was to identify allergenic proteins in garlic and to investigate their importance in allergies to other Allium species (leek, shallot, and onion).
Methods |
A crude extract of garlic proteins was separated by SDS-PAGE and 2-dimensional electrophoresis; immunoblotting was then performed with the use of individual and pooled sera from patients with garlic allergy, and the major IgE-binding proteins were analyzed by amino acid sequencing and mass spectrometry. The putative allergens were further purified by chromatography; the antigenicity, allergenicity, and IgE-binding cross-reactivity of the purified protein were then studied by immunoblotting, periodate oxidation, skin tests, and IgE-binding inhibition assays.
Results |
A major allergen, alliin lyase, was identified by mass spectrometry and Edman sequencing and purified to homogeneity through the use of a simple 2-step chromatographic method. Skin tests showed that the purified protein elicited IgE-mediated hypersensitive responses in patients with garlic allergy. Periodate oxidation showed that carbohydrate groups were involved in the antigenicity, allergenicity, and cross-reactivity. Garlic alliin lyase showed strong cross-reactivity with alliin lyases from other Allium species, namely leek, shallot, and onion.
Conclusions |
Alliin lyase was found to be a major garlic allergen in a garlic-allergic group of patients in Taiwan. The wide distribution of alliin lyase in Allium suggests it may be a new cross-reactive allergen.
Il testo completo di questo articolo è disponibile in PDF.Keywords : Proteomics, allergen, lectin-binding reactivity, cross-reactivity, alliin lyase, garlic, Allium sativum
Abbreviations : MALDI-TOF-MS, PSD, PVDF, TBST
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Supported in part by grant NSC-90-2320-B-002-138 from the National Science Council and grant VGH91-345 from the Veterans General Hospital-Taipei, Taiwan. |
Vol 113 - N° 1
P. 161-168 - Gennaio 2004 Ritorno al numeroBenvenuto su EM|consulte, il riferimento dei professionisti della salute.
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