Co-exposure to different bacterial species’ lipopolysaccharides with the NASH diet exacerbates NASH and liver fibrosis progression in mice - 17/10/24
Highlights |
• | This study simulates gut microbiota imbalance effects on NASH and liver fibrosis. |
• | GAN diet plus LPS treatment rapidly induces liver fibrosis. |
• | This model may facilitate the identification of gut microbiota species that influence liver fibrosis development in their hosts. |
Abstract |
Background and aim |
With the obesity epidemic, nonalcoholic fatty liver disease (NAFLD) has become a public health concern, but its progression mechanism remains unclear. Experimental models mimicking human NAFLD/steatohepatitis (NASH) are crucial. This study simulates gut microbiota imbalance effects on NASH and liver fibrosis.
Methods |
We used different bacterial sources of lipopolysaccharide (LPS), including Escherichia coli (GEC) and Salmonella abortus equi (GSE), combined with a Gubra Amylin NASH (GAN) diet to induce NASH and liver fibrosis.
Results |
The GSE group showed significantly higher serum alanine aminotransferase, hydroxyproline, CD68-positive cells, α-smooth muscle actin, glial fibrillary acidic protein, and TNF-α, COL1A1, TGF-β, and NLRP3 expressions compared to the the GAN group. The GSE group also had higher Erysipelotrichaceae, Akkermansiaceae, and Bacteroidaceae family numbers.
Conclusions |
The GAN diet with LPS treatment successfully induced NASH and fibrosis making this model useful for preclinical NASH drug testing.
Il testo completo di questo articolo è disponibile in PDF.Keywords : Nonalcoholic steatohepatitis, LPS, Hepatic inflammatory, Fibrosis, Gut microbiota
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Vol 48 - N° 9
Articolo 102470- Novembre 2024 Ritorno al numero
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