IL-17A mediates pyroptosis via the ERK pathway and contributes to steroid resistance in CRSwNP - 04/08/22
, Ge-Hua Zhang, MD, PhD a, ⁎ Abstract |
Background |
Pyroptosis is closely related to inflammation. However, the molecular mechanisms and pathologic contributions of pyroptotic epithelial cell are not yet fully understood.
Objective |
This study aimed to explore the function and molecular mechanisms of IL-17A on human nasal epithelial cell (hNEC) pyroptosis.
Methods |
The expression of pyroptosis-related biomarkers and IL-17A was assessed in sinonasal mucosa from control individuals, patients with chronic rhinosinusitis without nasal polyps, and patients with chronic rhinosinusitis with nasal polyps (CRSwNP) by using quantitative RT-PCR. Their localization was analyzed via immunohistochemistry and immunofluorescence. The ultrastructural characteristics of IL-17A–induced pyroptosis in hNECs were visualized by using electron microscopy. IL-17A functional assays were performed on hNECs and airway epithelial cell lines. Cytokine levels were quantified via ELISA. The signaling pathways involved in IL-17A–induced pyroptosis were studied via unbiased RNA sequencing and Western blotting.
Results |
The expression of IL-17A and the pyroptotic biomarkers NOD-like receptor family, pyrin domain containing 3 (NLRP3), caspase-1, gasdermin D, and IL-1β was increased in nasal mucosa from patients with CRSwNP compared with in those with chronic rhinosinusitis without nasal polyps and the control subjects. IL-17A was positively correlated and colocalized with the pyroptotic biomarkers. IL-17A treatment induced pyroptosis in the hNECs and cell lines analyzed, primarily through the extracellular signal–regulated kinase (ERK)-NLRP3/caspase-1 signaling pathway, and increased IL-1β and IL-18 secretion in hNECs. Moreover, IL-17A–induced pyroptosis contributed to steroid resistance by affecting glucocorticoid receptor-α and glucocorticoid receptor-β expression, and the inhibition of pyroptotic proteins partially abolished IL-17A–induced steroid resistance in hNECs.
Conclusion |
Elevated IL-17A level promotes pyroptosis in hNECs through the ERK-NLRP3/caspase-1 signaling pathway and contributes to glucocorticoid resistance by affecting glucocorticoid receptor homeostasis in patients with CRSwNP.
Il testo completo di questo articolo è disponibile in PDF.Graphical abstract |
Key words : Epithelial cells, pyroptosis, IL-17A, NLRP3 inflammasome, steroid resistance
Abbreviations used : ASC, C-Caspase-1, CRS, CRSsNP, CRSwNP, CT, DEG, ERK, GSDMD, GR, GRα, GRβ, hNEC, IF, IHC, IκBα, JNK, MAPK, MEK, NF-κB, NLRP3, NP, p, qRT-PCR, siRNA, VAS
Mappa
| The first 3 authors contributed equally to the completion of this article. |
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| Supported by the National Natural Science Foundation of China (grants 81970859, 82071020, and 82171114), the Natural Science Foundation of Guangdong Province, China (grant 2019A1515011093), and the Science and Technology Program of Guangzhou, China (grant 202002030034). |
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| Disclosure of potential conflict of interest: The authors declare that they have no relevant conflicts of interest. |
Vol 150 - N° 2
P. 337-351 - Agosto 2022 Ritorno al numero
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