A substantial neutrophilic inflammation as regular part of severe type 2 chronic rhinosinusitis with nasal polyps - 09/01/21
Abstract |
Background |
Chronic rhinosinusitis with nasal polyps (CRSwNP) is generally associated with severe type 2 immune reactions in the white population. However, recent findings suggest an additional role for neutrophils in severe type 2 inflammation.
Objective |
This study aimed to characterize the neutrophilic inflammation in CRSwNP and its relation to eosinophilic inflammation in severe type 2 immune reactions.
Methods |
The presence and activation of neutrophils and eosinophils was analyzed in CRS without NP and CRSwNP by measuring cell and activation markers via immunohistochemistry, immunofluorescence, Luminex assay, ELISA, UniCAP, fluorescence-activated cell sorting, and PCR. Differential neutrophil migration was assessed via Boyden-chamber assay and neutrophil survival was analyzed via flow cytometry.
Results |
Both CRS without NP and CRSwNP displayed variable degrees of eosinophilic and neutrophilic inflammation, with a profound neutrophilic infiltration and activation in type 2 CRSwNP, associated with eosinophil extracellular traps cell death and Charcot-Leyden crystals, but independent of IL-17. Neutrophil extracellular traps cell death in CRSwNP was associated with bacterial colonization, however, neutrophils were less prone to undergo neutrophil extracellular traps cell death in the tissue of patients with severe type 2 CRSwNP. Neutrophils did not show increased migration nor survival in the CRSwNP environment in vitro.
Conclusions |
This study demonstrated a severe neutrophilic inflammation associated with severe eosinophilic type 2 inflammatory CRSwNP, the role of which needs further study.
Il testo completo di questo articolo è disponibile in PDF.Key words : CRSsNP, CRSwNP, type 2 inflammation, neutrophils, NETosis, IL-17, Charcot-Leyden crystals
Abbreviations used : CitH3, CLC, CRS, CRSsNP, CRSwNP, EETosis, Gal10, MBP, NETosis
Mappa
E.G. was supported by a post-doctoral research fellowship from the Fonds Wetenschappelijk Onderzoek (FWO) (FWO/DPO/108). C.B. and E.G. were supported by grants from FWO Flanders (1515516N, EOS GOG2318N), the Interuniversity Attraction Poles Grant P7/30, and Sanofi (A17/TT/1942, A19/TT/0828). |
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Disclosure of potential conflict of interest: The authors declare that they have no relevant conflicts of interest. |
Vol 147 - N° 1
P. 179 - Gennaio 2021 Ritorno al numeroBenvenuto su EM|consulte, il riferimento dei professionisti della salute.
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