Proteomic analysis of nasal mucus samples of healthy patients and patients with chronic rhinosinusitis - 09/01/21
Abstract |
Background |
Chronic rhinosinusitis (CRS) has a complex and multifactorial pathogenesis with a heterogeneous inflammatory profile. Proteomic analysis of nasal mucus may enable further understanding of protein abundances and biologic processes present in CRS and its endotypes compared with in healthy patients.
Objective |
Our aim was to determine differences in the nasal mucus proteome of healthy patients and patients with CRS.
Methods |
Nasal mucus was obtained from healthy patients, patients with CRS without nasal polyps (CRSsNP), and patients with CRS with nasal polyps (CRSwNP) before surgery. Gel electrophoresis was performed to fractionate the complex protein extracts before mass spectrometry analysis. Gene set enrichment analysis was performed on differentially expressed proteins.
Results |
A total of 33 patients were included in this study (12 healthy, 10 with CRSsNP, and 11 with CRSwNP). In all, 1142 proteins were identified in mucus samples from healthy patients, 761 in mucus samples from patients with CRSsNP, and 998 in mucus samples from patients with CRSwNP. Dysfunction in immunologic pathways, reduced cellular signaling, and increased cellular metabolism with associated tissue remodeling pathways were present in patients with CRS compared with in healthy patients.
Conclusion |
Significant downregulation of mucosal immunity and antioxidant pathways with increased tissue modeling processes may account for the clinical manifestations of CRS. Ultimately, the differing proteome and biologic processes provide further insight into CRS pathogenesis and its endotypes.
Il testo completo di questo articolo è disponibile in PDF.Graphical abstract |
Key words : Nasal mucus, proteomics, chronic rhinosinusitis, nasal polyps, endotypes, proteins
Abbreviations used : CRS, CRSsNP, CRSwNP, emPAI, GO, GSEA, MS, ROS
Mappa
| This project was supported by the Adelaide University Research Training Program Scholarship, Bertha Sudholz Research Scholarship, and Garnett Passe Rodney Williams Memorial Foundation Scholarship. |
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| Disclosure of potential conflict of interest: P.-J. Wormald receives royalties from Medtronic, Integra, and Scopis, and he is a consultant for NeulMed. A. J. Psaltis is a consultant for Tissium, Medtronic, and ENT Technologies. The rest of the authors declare that they have no relevant conflicts of interest. |
Vol 147 - N° 1
P. 168-178 - Gennaio 2021 Ritorno al numero
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