Gal d 7—a major allergen in primary chicken meat allergy - 22/07/20
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Abstract |
Background |
Poultry meat can induce severe allergic reactions. So far, the molecules causing poultry meat allergy are largely unknown.
Objective |
Our aim was to identify and characterize poultry meat allergens.
Methods |
Profiles of patients’ IgE reactivity to chicken muscle were analyzed in immunoblots, and proteins recognized by the majority of patients were subjected to peptide mass fingerprinting. A 23-kDa IgE-reactive protein was identified as myosin light chain 1, designated Gallus domesticus 7 (Gal d 7). Recombinant Gal d 7 was produced in Escherichia coli. The protein’s IgE reactivity was analyzed in ELISA experiments, and cross-reactivity with allergens of other poultry species was assessed in inhibition immunoblots. Fold and thermal stability were evaluated by circular dichroism analysis, and enzymatic stability was investigated using in vitro gastrointestinal digestion assays.
Results |
Recombinant Gal d 7 represents a properly folded, predominantly α-helical protein and displays IgE-binding activity comparable to that of its natural counterpart. IgE reactivity analysis in 28 patients allergic to chicken meat revealed that Gal d 7 is a major allergen for patients primarily sensitized to chicken meat. Furthermore, Gal d 7–cross-reactive allergens were also detected in other poultry species, suggesting that recombinant Gal d 7 can be used as a diagnostic marker allergen for poultry meat allergy. The high thermal stability, refolding capacity, and resistance to gastrointestinal enzymes might explain why Gal d 7 can act as a potent sensitizing agent.
Conclusion |
Gal d 7 represents a novel major chicken meat allergen. Recombinant Gal d 7 could be used for diagnosis of genuine poultry meat sensitization.
Il testo completo di questo articolo è disponibile in PDF.Graphical abstract |
Key words : Poultry meat allergy, major chicken meat allergen, recombinant allergen, Gal d 7, genuine sensitization
Abbreviations used : CD, Gal d 5, Gal d 7, IMAC, LC-ESI MS/MS, MALDI-TOF, MLC, rGal d 7, SGF, SIF, SSF
Mappa
This study was supported by research grants P 25868-B13 and P 29991 from the Austrian Science Fund Austria and research grant 15-10 from the Municipal Department 23 for Economic Affairs, Labor and Statistics, Vienna, Austria. |
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Disclosure of potential conflict of interest: The authors declare that they have no relevant conflict of interest. |
Vol 146 - N° 1
P. 169 - Luglio 2020 Ritorno al numeroBenvenuto su EM|consulte, il riferimento dei professionisti della salute.
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