HLA class I and class II alleles and haplotypes of Algerian population from Algiers and neighbouring area - 13/12/24

Allèles et haplotypes HLA de classe I et de classe II dans la population algérienne d’Alger et des environs

Doi : 10.1016/j.tracli.2024.11.007

Malika Bouali-Benhalima ^a,^c,^⁎ , Meriem Abbadi ^a,^c, Sarah Hamada ^b, Celine Mandier ^b, Souhila Amoura ^a, Valérie Dubois ^b, Sofiane Samir Salah ^a,^c
^a Immunology Department, Mustapha Bacha University Hospital, Algiers, Algeria
^b HLA Laboratory Etablissement Français du Sang Auvergne Rhone Alpes, Lyon, France
^c University of Health Sciences, Algiers, Algeria

^⁎Corresponding author.

En prensa. Pruebas corregidas por el autor. Disponible en línea desde el Friday 13 December 2024

Highlights

•	This work is bringing new knowledge about allele frequency and haplotype segregation in the Algerian population.
•	The comparison with other described populations can bring an idea about population migration and closest of the studied population to another.
•	This work had been performed in the Histocompatibility laboratory of CHU Mustapha in Algiers. It was managed by Pr Benhalima.

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Abstract

In this study, we aimed to investigate the current genetic diversity and provide additional insights into the origins of the Algerian population by analyzing the frequencies of HLA -A,-B,-DRB1,-DQB1 alleles and associated haplotypes.

We analyzed 1,082 unrelated healthy Algerian individuals, who were potential kidney donors, recruited and assessed in the Immunology Department of CHU Mustapha in Algiers over a 10-year period (2009–2019). HLA genotyping was performed by Polymerase Chain Reaction Sequence Specific Primers (PCR-SSP). The allelic typing estimate and haplotype frequencies were calculated using the R studio® software and Haplotype frequency estimation from the website hla-net.eu/.

We identified a total of 20 HLA-A alleles, 31 HLA-B alleles, 13 HLA-DRB1 alleles and5 HLA-DQB1 alleles.

Regarding the allele frequency, the haplotype frequency and the distribution with other population database, we were able to provide a description of Algerian genetic background and draw some anthropogenetic conclusions.

This study of HLA polymorphism in the Algerian population shows a strong genetic similarity with the populations of southern Europe and North Africa (Morocco, Tunisia and Libya) with which it has shared a common history since antiquity.

In addition to its contribution to anthropogenetics, our study, serve as a reference database for studies exploring associations between the HLA system and certain pathologies.

El texto completo de este artículo está disponible en PDF.

Keywords : Algeria, Alleles, Haplotypes, HLA