Comparative evaluation of oxidative and biochemical parameters of red cell concentrates (RCCs) prepared from G6PD deficient donors and healthy donors during RCC storage - 30/10/24
, Ramazan Ali Khavari-Nejad a, Mahdieh Meschi cHighlights |
• | Glucose-6-phosphate dehydrogenase (G6PD) as an important antioxidant enzyme in RBC and has a protective effect against oxidative stress. |
• | G6PD deficiency is the most inherited enzyme disorders in red blood cell (RBC) and its lack weakens red blood cells against oxidative stress. |
• | Oxidative damage in red cell concentrates prepared from G6PD deficient donors was more considerable than healthy donors during storage and subsequently, the RBC storage lesion is also higher in G6PD deficient donors than in healthy donors. |
• | Screening of G6PD enzyme deficiency in pandemic area before blood transfusion seems to be necessary and it will help to improve the quality of donated blood in the blood transfusion centers. |
Abstract |
Introduction |
Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common inherited enzyme disorder in red blood cell (RBC). Due to the importance of G6PD enzyme as an antioxidant in RBC, we tried to investigate the oxidative damage in red cell concentrates (RCCs) prepared from donors with G6PD enzyme deficiency in comparison with healthy donors.
Material method |
This cross-sectional study was conducted on 20 male donors. Ten of the donors had G6PD deficiency (as a case) and the others had normal enzyme activity (as a control). Biochemical and oxidative damage parameters were examined in RCCs prepared from two groups on days 0, 7, 14, 21, 28 and 35 of RCCs storage; data comparison was analyzed by SPSS statistical software.
Results |
According to the result, lactate concentration increased significantly from the 7th day to the 35th day of RCC storage in G6PD-deficient donors compared to the control (P < 0.05). In addition, malondialdehyde (MDA) concentration in G6PD-deficient RCC showed a significant increase compared to the control in all days of storage (P < 0.05). Among the hematological parameters, mean corpuscular volume (MCV) and mean cell hemoglobin (MCH) increased significantly in all days of RCC storage in G6PD-deficient donors compared to the control (P < 0.05).
Conclusion |
Our study showed that oxidative changes in G6PD-deficient donors were significantly increased compared to the healthy donors, which probably leads to RCC storage lesion and an increase in blood transfusion complications. Due to the high prevalence of G6PD enzyme deficiency in pandemic areas, it seems that enzyme screening should be included in donor screening programs.
El texto completo de este artículo está disponible en PDF.Keywords : G6PD deficiency, Oxidative damage, Red cell concentrate, Red cell storage, Blood transfusion
Esquema
Vol 31 - N° 4
P. 201-208 - novembre 2024 Regresar al número
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