Dental pulp stem cell transplantation facilitates neuronal neuroprotection following cerebral ischemic stroke - 18/06/22
, Mingchang Li a, ⁎ Abstract |
Objectives |
This study aimed to identify and evaluate the intracranial transplantation of dental pulp stem cells (DPSCs) as a possible ischemic stroke therapy that mitigates neuronal death/apoptosis.
Materials and methods |
DPSCs were isolated from the impacted third molars of healthy volunteers and then intracranially injected at 24 h post-ischemic stroke to Sprague Dawley rats that had been subjected to 2 h of middle cerebral artery occlusion. Neurological functional deficits were assessed using the modified neurological severity score (mNSS), and cerebral edema was quantified using brain water content. Neuronal death/apoptosis was indicated by TdT-mediated dUTP Nick-End Labeling (TUNEL) staining, NeuN immunofluorescence and immunohistochemistry, and Western blot analysis of the protein expression of anti-apoptotic indicator of Bcl-2 and apoptotic indicators of Bax and caspase 3.
Results |
DPSC transplantation could ameliorate neurological dysfunction and brain edema, reduce infarct volume, decrease the percentage of TUNEL-positive nuclei, increase the number and percentage of NeuN-positive cells in ischemic penumbra, increase the ratio of Bcl-2 and Bax and down-regulate the production of caspase 3 in the cortical infarct zone.
Conclusions |
DPSC therapy via intracranial injection exerted remarkably neuroprotection mainly by inhibiting neuronal death/apoptosis.
El texto completo de este artículo está disponible en PDF.Highlights |
• | Dental pulp stem cell (DPSC) implantation by intracranial injection was effective for ischemic stroke treatment. |
• | Transplantation of 1 × 105 DPSCs was feasible and functional for ischemic stroke treatment. |
• | DPSC implantation at 24 h after ischemic stroke was effective for ischemic stroke treatment. |
• | DPSC implantation attenuated ischemic injury through inhibiting neuronal apoptosis and reducing brain edema. |
Abbreviations : DPSCs, MSCs, BM-MSCs, AD-MSCs, NGF, BDNF, VEGF, tMCAO, ICA, TTC, TUNEL, mNSS, BBB, BMVECs, TJs
Keywords : Dental pulp stem cells, Transplantation, Ischemic stroke, Neuroprotection, Neuronal survival
Esquema
Vol 152
Artículo 113234- août 2022 Regresar al número
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