RFamide-related peptide (RFRP-3), the Mammalian ortholog of the Avian gonadotropin-inhibitory hormone (GnIH), is a novel neuropeptide known for its inhibitory regulatory effect on reproduction in various mammalian species. However, a stimulatory action has been reported. This paper aims to: i) study the histology of the epididymis (caput) of Gerbillus tarabuli during the breeding period; and ii) to determine the distribution of the “RFRP-3/receptors system” in the epididymis (caput) of this desert rodent during the active season, and thus, to inspect its potential local interfering in sperm maturation. For that, immunohistochemistry was performed to detect the epididymal immunolocalizations of the three molecules, RFRP-3, GPR147, and GPR74. This is the first report of the epididymis histology in Gerbillus tarabuli, as it is the first evidence of the existence of the RFRP-3/Receptor system in the same organ of the same species. During the breeding season, moderate immunostaining of the RFRP-3/receptors system was present in the caput epididymis’ epithelial parts (basal and principal cells) and spermatozoa. In contrast, these three molecules were absent in the peritubular and muscle coat's myoid cells and of the interstitial part of the caput epididymis. The results suggest that the epididymis is a potential source of RFRP-3 in the desert Rodent, Gerbillus tarabuli, which may function as a paracrine and/or autocrine factor affecting the main epididymis’ function: sperm maturation.