Impacts of chemically different surfaces of implants on a biological activity of fibroblast growth factor-2–apatite composite layers formed on the implants - 30/01/21
, Kengo Fujii b, Yohei Yanagisawa b, Fumiko Kobayashi b, Shinji Murai b, Hirotaka Mutsuzaki c, Yuki Hara b, Masashi Yamazaki b, Atsuo Ito a
Bienvenido a EM-consulte, la referencia de los profesionales de la salud.
Artículo gratuito.
Conéctese para beneficiarse!
Abstract |
Background |
Implants coated with fibroblast growth factor-2 (FGF-2)–apatite composite layers were previously reported to enhance soft-tissue formation, bone formation, and angiogenesis around the implants owing to the biological activity of FGF-2. However, it is unclear whether the chemistries of the material and surface of implants have some impact on the retention of the biological activity of FGF-2 in FGF-2−apatite composite layers on them. Since magnitude of the impact should be evaluated for extensive application of the composite layer to coat various implants, following items were examined; (1) surface chemistries of six implants, (2) mitogenic activities of FGF-2 in FGF-2–apatite composite layers on the implants, and (3) improved synthesis method of the composite layer for retention of the mitogenic activity of FGF-2.
Hypothesis |
The biological activity of FGF-2 in the composite layer is affected by the chemistries of the material and surface of implants.
Materials and methods |
Six commercial products of pins and screws having different surface chemistries were coated with FGF-2–apatite composite layers. The composite layers were quantitatively analyzed for calcium (Ca), phosphorus (P) and FGF-2, and also evaluated the mitogenic activities of FGF-2. Improvement of the synthesis method was then attempted using two pin products.
Results |
Each commercial product had a chemically and morphologically characteristic surface. FGF-2–apatite composite layers were formed on all the commercial products. Although the Ca, P, and FGF-2 contents (4.7±0.9μg/mm, 2.2±0.4μg/mm, and 21.1±3.7ng/mm, respectively) and the Ca/P molar ratios (1.69±0.01) of the composite layers were almost the same, rate of retention of the mitogenic activity of FGF-2 in the composite layers significantly decreased on some pin products (3/12–4/12). The decrease in rate of retention of the mitogenic activity of FGF-2 was prevented by a two-step synthesis method to form a composite layer on a precoating with calcium phosphate (9/12–12/12).
Discussion |
The chemistries of the implant surfaces had a significant impact on the retention of the mitogenic activity of FGF-2 in the composite layers formed on the implant. The two-step synthesis method was useful to retain mitogenic activity of FGF-2 regardless of the surface chemistries of the implants. The two-step synthesis method has potential to expand the applicability of FGF-2–apatite composite layers to a wider range of implants.
Level of evidence |
III, Case control in vitro study.
El texto completo de este artículo está disponible en PDF.Keywords : Implant surfaces, Apatite, Fibroblast growth factor-2 (FGF-2), Mitogenic activity
Esquema
Vol 107 - N° 1
Artículo 102748- février 2021 Regresar al número
Artículo precedente
- Do custom 3D-printed revision acetabular implants provide enough value to justify the additional costs? The health-economic comparison of a new porous 3D-printed hip implant for revision arthroplasty of Paprosky type 3B acetabular defects and its closest alternative
- Philip Tack, Jan Victor, Paul Gemmel, Lieven Annemans
- Cementless hip revision cup for the primary fixation of osteoporotic acetabular fractures in geriatric patients
- Christopher A. Becker, Christoph Linhart, Jan Bruder, Christian Zeckey, Axel Greiner, Adrian Cavalcanti Kußmaul, Simon Weidert, Eduardo M. Suero, Wolfgang Böcker, Christian Kammerlander
Bienvenido a EM-consulte, la referencia de los profesionales de la salud.