Long non-coding RNA MBNL1-AS1 regulates proliferation, migration, and invasion of cancer stem cells in colon cancer by interacting with MYL9 via sponging microRNA-412-3p - 29/01/20
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Highlights |
• | This study explores the role of MBNL1-AS1 in colon cancer via miR-412-3p and MYL9. |
• | MBNL1-AS1 and MYL9 is downregulated in colon cancer. |
• | MBNL1-AS1 acts as a sponge of miR-412-3p and negatively regulates miR-412-3p. |
• | MBNL1-AS1 mediates colon cancer stem cell properties by sponging miR-412-3p. |
• | This study provides a promising therapeutic strategy for colon cancer treatment. |
Summary |
Background/Aims |
Colon cancer is a common cancer that is a threat to human health. Some long non-coding RNAs (lncRNAs) have been observed to exert roles in colon cancer. Here, the current study is aimed to explore the potential mechanism of lncRNA MBNL1 antisense RNA 1 (MBNL1-AS1) in progression of colon cancer and the associated mechanisms.
Methods |
Microarray analysis was performed to screen differentially expressed lncRNA and genes associated with colon cancer and its potential mechanism. The functional role of MBNL1-AS1 in colon cancer was analyzed, followed identification of the interaction among MBNL1-AS1, microRNA-412-3p (miR-412-3p), and MYL9. Subsequently, CSC viability, migration, invasion, and apoptosis were detected though a series of in vitro experiments. At last, in vivo experiments were performed to assess tumor formation of colon CSCs.
Results |
MBNL1-AS1 and MYL9 were poorly expressed in colon cancer. MBNL1-AS1 could competitively bind to miR-412-3p so as to promote MYL9 expression. Enhancement of MBNL1-AS1 or inhibition of miR-412-3p was shown to decrease CSC proliferation, migration, and invasion but promote apoptosis. Moreover, MBNL1-AS1 reversed the CSC-like properties as well as xenograft tumor formation in vivo induced by miR-412-3p.
Conclusion |
Collectively, the present study suggests an inhibitory role of MBNL1-AS1 in colon cancer by upregulating miR-412-3p-targeted MYL9. Thus, this study provides an enhanced understanding of MBNL1-AS1 along with miR-412-3p and MYL9 as therapeutic targets for colon cancer.
El texto completo de este artículo está disponible en PDF.Keywords : Colon Cancer, Long non-coding RNA MBNL1-AS1, MicroRNA-412-3p, MYL9, Cancer stem cells
Esquema
Vol 44 - N° 1
P. 101-114 - février 2020 Regresar al número
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