Genetic diversity and drug susceptibility profile of Mycobacterium tuberculosis isolated from different regions of India - 30/06/15

Doi : 10.1016/j.jinf.2015.04.028

Jitendra Singh ^a, Manimuthu Mani Sankar ^a, Parveen Kumar ^a, David Couvin ^b, Nalin Rastogi ^b, Sarman Singh ^a,^⁎
Indian TB Diagnostics Network^{^c}
The Other members of the Indian TB Diagnostics Network are: V.M. Katoch (the then Director of National JALMA Institute for Leprosy Other Mycobacterial Diseases, Agra and presently Director General of Indian Council of Medical Research, New Delhi); DS Chauhan & Kiran Katoch (National JALMA Institute for Leprosy Other Mycobacterial Diseases, Agra); Camila Rodrigues (PD Hinduja Hospital & Research Institute, Mumbai), V. Lakshmi (Nizam's Institute of Medical Sciences, Hyderabad); GM Taori, HF Daginawala & Rajpal Singh (Central India Institute of Medical Sciences, Nagpur), Basudev Bhattacharya (Institute of Post Graduate Medical Education & Research, Calcutta); Bandana Choudhury (Intermediate Reference Laboratory, Gauhati Medical College, Guwahati, Assam); Niti Singh (National Institute of TB and Respiratory Diseases, New Delhi); Uma Devi & Soumya Swaminathan (National Institute of Research in Tuberculosis, Chennai).
V.M. Katoch ^c,^d, D.S. Chauhan ^c, Kiran Katoch ^c, Camila Rodrigues ^e, V. Lakshmi ^f, G.M. Taori ^g, H.F. Daginawala ^g, Rajpal Singh ^g, Basudev Bhattacharya ^h, Bandana Choudhury ⁱ, Niti Singh ^j, Uma Devi ^k, Soumya Swaminathan ^k
^c National JALMA Institute for Leprosy Other Mycobacterial Diseases, Agra, India
^d Indian Council of Medical Research, New Delhi, India
^e PD Hinduja Hospital & Research Institute, Mumbai, India
^f Nizam's Institute of Medical Sciences, Hyderabad, India
^g Central India Institute of Medical Sciences, Nagpur, India
^h Institute of Post Graduate Medical Education & Research, Calcutta, India
ⁱ Intermediate Reference Laboratory, Gauhati Medical College, Guwahati, Assam, India
^j National Institute of Tuberculosis and Respiratory Diseases, New Delhi, India
^k National Institute of Research in Tuberculosis, Chennai, India

^a Division of Clinical Microbiology and Molecular Medicine, Department of Laboratory Medicine, All India Institute of Medical Sciences, New Delhi, India
^b WHO Supranational TB Reference Laboratory, Institute Pasteur de la Guadeloupe, Abymes, Guadeloupe, France

^∗Corresponding author. Division of Clinical Microbiology and Molecular Medicine, Department of Laboratory Medicine, All India Institute of Medical Sciences, New Delhi 110029, India. Tel.: +91 11 26588484; fax: +91 11 26588663.

Summary

Objectives

Molecular genotyping profiles of Mycobacterium tuberculosis (MTB) provide a valuable insight into the evolution and transmission of the bacilli. Due to the lack of comprehensive national level data from India on this subject, we performed this study to determine the recent trends and distribution of various MTB lineages circulating in India.

Methods

A total of 628 MTB isolates were obtained from North, West, South, Central and Eastern India. Spoligotyping and drug susceptibility testing was performed by using manufacturer's instructions.

Results

Spoligotyping detected 102 distinct spoligo-patterns. A total of 536 (85.3%) isolates were distributed into 85 SITs which matched the pre-existing database, whereas 17 SITs were newly created for 34 (5.4%) isolates. Overall, CAS family genotype was predominant, comprising 222 (35.4%) isolates, followed by EAI in 152 (24.2%), Beijing in 108 (17.2%), Manu in 41 (6.5%), T in 30 (4.8%), H in 6 (0.9%), X in 3 (0.5%) and one (0.2%) each in Ural and AFRI. Drug susceptibility testing identified 134 (21.3%) isolates as multi drug resistant (MDR).

Conclusions

The CAS lineage had a pan India presence but EAI lineage was confined to southern parts of India. Beijing genotype of MTB was significantly associated (p-value <0.0001) with MDR.

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Highlights

•	Genotyping of MTB is a valuable tool to understand its evolution and transmission.
•	A total of 628 MTB isolates obtained from various parts of India were spoligotyped.
•	A total of 536 (85.3%) isolates were distributed into 85 SITs whereas 17 SITs were newly created for 34 (5.4%) isolates.
•	CAS family genotype was predominant across India but EAI lineage was confined mainly to south India.
•	Beijing was more associated (p-value <0.0001) with MDR.

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Keywords : MTB, Spoligotyping, India

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Vol 71 - N° 2

P. 207-219 - août 2015 Retour au numéro

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Genetic diversity and drug susceptibility profile of Mycobacterium tuberculosis isolated from different regions of India - 30/06/15

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