Genotype shift in human coronavirus OC43 and emergence of a novel genotype by natural recombination - 08/05/15
, Jianwei Wang a, ⁎
Bienvenue sur EM-consulte, la référence des professionnels de santé.
Article gratuit.
Connectez-vous pour en bénéficier!
Summary |
Background |
Human coronavirus (HCoV) OC43 is the most prevalent HCoV in respiratory tract infections. Its molecular epidemiological characterization, particularly the genotyping, was poorly addressed.
Methods |
The full-length spike (S), RNA-dependent RNA polymerase (RdRp), and nucleocapsid (N) genes were amplified from each respiratory sample collected from 65 HCoV-OC43-positive patients between 2005 and 2012. Genotypes were determined by phylogenetic analysis. Recombination was analyzed based on full-length viral genome sequences. Clinical manifestations of each HCoV genotype infection were compared by reviewing clinical records.
Results |
Sixty of these 65 samples belong to genotypes B, C and D. The remaining five strains had incongruent positions in the phylogenetic trees of the S, RdRp and N genes, suggesting a novel genotype emerging, designated as genotype E. Whole genome sequencing and bootscan analysis indicated that genotype E is generated by recombination between genotypes B, C and D. Temporal analysis revealed a sequential genotype replacement of C, B, D and E over the study period with genotype D being the dominant genotype since 2007. The novel genotype E was only detected in children younger than three years suffering from lower respiratory tract infections.
Conclusions |
Our results suggest that HCoV-OC43 genotypes are evolving. Such genotype shift may be an adapting mechanism for HCoV-OC43 maintaining its epidemic.
Le texte complet de cet article est disponible en PDF.Graphical abstract |
Highlights |
• | Temporal shift of multiple human coronavirus OC43 genotypes. |
• | Emergence of a novel genotype E by natural recombination. |
• | Genotype D dominated HCoV-OC43 epidemic in China in recent years. |
• | Genotype evolving plays an important role in HCoV-OC43 epidemic. |
Keywords : Human coronavirus OC43, Respiratory infection, Molecular epidemiology, Genotype, Recombination
Plan
Vol 70 - N° 6
P. 641-650 - juin 2015 Retour au numéro
Article précédent
- Cellular microRNA-miR-548g-3p modulates the replication of dengue virus
- Weitao Wen, Zhenjian He, Qinlong Jing, Yiwen Hu, Cuiji Lin, Rui Zhou, Xiaoqun Wang, Yangfan Su, Jiehao Yuan, Zhenxin Chen, Jie Yuan, Jueheng Wu, Jun Li, Xun Zhu, Mengfeng Li
- HIV-1 induces in vivo platelet activation by enhancing platelet NOX2 activity
- Daniele Pastori, Antonella Esposito, Roberto Carnevale, Simona Bartimoccia, Marta Novo, Alessandra Fantauzzi, Francesco Di Campli, Pasquale Pignatelli, Francesco Violi, Ivano Mezzaroma
Bienvenue sur EM-consulte, la référence des professionnels de santé.