Transcriptomic correlates of organ failure extent in sepsis - 11/04/15
, María Heredia-Rodríguez a, c , Esther Gomez-Sanchez a, c , David Andaluz-Ojeda a, d , Verónica Iglesias a, b , Lucia Rico a, b , Alicia Ortega a, b , Estefanía Gomez-Pesquera a, c , Pilar Liu a, c , Marta Aragón a, c , Jose Maria Eiros e , Maria Ángeles Jiménez-Sousa a, f , Salvador Resino a, f , Ignacio Gómez-Herreras a, c , Jesús F. Bermejo-Martín a, b, ⁎, g , Eduardo Tamayo a, c, g Summary |
Objectives |
Sepsis is characterised by the frequent presence of organ failure and marked immunologic alterations. We studied the association between the extent of organ failure and the transcriptomic response of septic patients.
Methods |
Gene expression profiles in the blood of 74 surgical patients with sepsis were compared with those of 30 surgical patients with no sepsis. Differentially expressed genes were assessed for their correlation with the sequential organ failure (SOFA) score.
Results |
The expression levels of a group of genes participating in the cell cycle (HIST1H1C, CKS2, CCNA2, CDK1, CCNB2, CIT, CCNB1, AURKA, RAD51), neutrophil protease activity (ELANE, ADORA3, MPO, MMP8, CTSG), IL-1R and IL-18R response correlated directly with SOFA and mortality. Genes involved in T cell (LCK, CD3G, CD3D, ZAP70, ICOS, CD3E, CD28, IL2RB, CD8B, CD8A, CD40LG, IL23A, CCL5, SH2D1A, ITK, CD247, TBX21, GATA3, CCR7, LEF1, STAT4) and NK cell immunity (CD244, KLRK1, KLRD1) were inversely associated with SOFA and mortality.
Conclusions |
The extent of organ failure in sepsis correlates directly with the existence of imbalanced innate and adaptive responses at the transcriptomic level. Quantification of the expression levels of the genes identified here could contribute to the simultaneous assessment of disease severity and immunological alterations in sepsis.
Le texte complet de cet article est disponible en PDF.Highlights |
• | Organ failure extent in sepsis is linked to gradual transcriptomic changes in blood. |
• | These changes involve mostly 55 genes of cell cycle, innate and adaptive immunity. |
• | These changes influence patients'outcome. |
• | Assessing them could help to evaluate both disease severity and immunity in sepsis. |
Keywords : Sepsis, Transcriptomics, Organ failure extent, SOFA, Immune response, Immunosupression, Microarrays
Plan
Vol 70 - N° 5
P. 445-456 - mai 2015 Retour au numéro
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