Adjunctive biomarkers for improving diagnosis of tuberculosis and monitoring therapeutic effects - 01/03/15
Summary |
Objectives |
To identify host biomarkers associated with latent tuberculosis infection (LTBI), active tuberculosis (TB), and nontuberculous mycobacteria (NTM) diseases to improve diagnosis and effective anti-TB treatment.
Methods |
Active TB and NTM patients at diagnosis, recent TB contacts, and normal healthy subjects were recruited. Tuberculin skin tests, QuantiFERON-TB Gold In-Tube tests, and multiplex bead arrays with 17 analytes were performed. TB patients were re-evaluated after 2 and 6 months of treatment.
Results |
Mycobacterium tuberculosis (M. tb) antigen-specific IFN-γ, IL-2, and CXCL10 responses were significantly higher in active TB and LTBI compared with controls (P < 0.01). Only serum VEGF levels varied between the active TB and LTBI groups (AUC = 0.7576, P < 0.001). Active TB and NTM diseases were differentiated by serum IL-2, IL-9, IL-13, IL-17, TNF-α and sCD40L levels (P < 0.05). Increased sCD40L and decreased M. tb antigen-specific IFN-γ levels correlated with sputum clearance of M. tb after 2 months of treatment (P < 0.001).
Conclusions |
Serum IL-2, IL-9, IL-13, IL-17, TNF-α, sCD40L and VEGF-A levels may be adjunctive biomarkers for differential diagnosis of active TB, LTBI, and NTM disease. Assessment of serum sCD40L and M. tb antigen-specific IFN-γ, TNF-α, and IL-2 levels could help predict successful anti-TB treatment in conjunction with M. tb clearance.
Le texte complet de cet article est disponible en PDF.Highlights |
• | Analytes from individuals with active TB, NTM, or LTBI, and controls were analysed. |
• | Serum VEGF-A has diagnostic value for distinguishing active TB from LTBI. |
• | Active TB and NTM diseases were differentiated by analyte concentrations in serum. |
• | Levels of sCD40L and IFN-γ correlated with M. tb sputum clearance after treatment. |
• | Measurement of multiple analytes may improve diagnosis of TB, LTBI and NTM disease. |
Keywords : Biomarker, Diagnosis, Latent tuberculosis infection (LTBI), Mycobacterium tuberculosis (M. tb), Nontuberculous mycobacteria (NTM), Tuberculosis (TB), Treatment
Plan
Vol 70 - N° 4
P. 346-355 - avril 2015 Retour au numéro
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