Human B cells produce chemokine CXCL10 in the presence of Mycobacterium tuberculosis specific T cells - 25/12/14
, Ahmed M. Salman b, 1 , Morten Ruhwald a , Pernille Ravn c , Inger Brock d , Nabila Elsheikh e , Peter Andersen a , Else Marie Agger a Summary |
Background |
The role of B cells in human host response to Mycobacterium tuberculosis (Mtb) infection is still controversial, but recent evidence suggest that B cell follicle like structures within the lung may influence host responses through regulation of the local cytokine environment. A candidate for such regulation could be the chemokine CXCL10. CXCL10 is mainly produced by human monocytes, but a few reports have also found CXCL10 production by human B cells. The objective of this study was to investigate CXCL10 production by human B cells in response to in vitro stimulation with Mtb antigens.
Methodology/principal findings |
We analyzed human blood samples from 30 volunteer donors using multiparameter flow cytometry, and identified a subgroup of B cells producing CXCL10 in response to in vitro stimulation with antigens. T cells did not produce CXCL10, but CXCL10 production by B cells appeared to be mediated via IFN-γ and dependent on contact with antigen-specific T cells recognizing the antigen.
Conclusion |
Human B cells are able to produce CXCL10 in an IFN-γ and T cell contact-dependent manner. The present findings suggest a possible mechanism through which B cells in part may influence granuloma formation in human tuberculosis (TB) and participate in infection control.
Le texte complet de cet article est disponible en PDF.Keywords : B cells, CXCL10, Peptides, Mycobacterium tuberculosis, Granuloma
Plan
Vol 95 - N° 1
P. 40-47 - janvier 2015 Retour au numéro
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