Analysis of miRNA expression profiling in human macrophages responding to Mycobacterium infection: Induction of the immune regulator miR-146a - 08/05/14
, Di Wang b, ⁎ Summary |
Objectives |
The regulatory mechanism of microRNA (miRNA) within macrophage innative response to Mycobacterium tuberculosis infection is not clear yet.
Methods |
The expression profile of cellular miRNAs during Mycobacterium bovis BCG infection was analyzed by using microarray. The expression of miR-146a was evaluated in alveolar macrophages (AMs) of bronchoalveolar lavage solution from pulmonary tuberculosis (PTB) patients and healthy volunteers respectively. Inhibitor experiment and promoter analysis were used to investigate the pathway involved in the induction of miR-146a. Examination of miR-146a function in macrophages was performed by overexpression and inhibition of miR-146a.
Results |
Among the altered miRNAs, 10 were downregulated whereas 8 were upregulated in M. bovis BCG-infected macrophage. MiR-146a was high expressed in cultured macrophage respond to M. bovis BCG but decreased in AMs of PTB patients, and stated a negative correlation with degree of smear-positive. Nuclear factor-κB pathway was required for the induction of miR-146a. Overexpression of miR-146a results in significant reduction of PTGS2 and enhanced the killing ability of THP-1 cells to intracellular M. bovis BCG, and miR-146a negatively regulated TNF-α release in feedback manner.
Conclusions |
Our findings suggest an important role of miR-146a in M. bovis BCG infection that helps to fine-tune the inflammation response of MTB infection.
Le texte complet de cet article est disponible en PDF.Keywords : miR-146a, Mycobacterium bovis BCG, Inflammation, PTGS2, TNF-α
Plan
Vol 68 - N° 6
P. 553-561 - juin 2014 Retour au numéro
Article précédent
- Daptomycin is effective as antibiotic-lock therapy in a model of Staphylococcus aureus catheter-related infection
- Yolanda Meije, Benito Almirante, José Luis Del Pozo, María Teresa Martín, Nuria Fernández-Hidalgo, Adriana Shan, Jana Basas, Albert Pahissa, Joan Gavaldà
- Immune responses to pneumococcal pilus RrgA and RrgB antigens and their relationship with pneumococcal carriage in humans
- Muhammad S. Ahmed, Stephen Derbyshire, Brian Flanagan, Christopher Loh, Max McCormick, Michele Barocchi, Vega Masignani, Adam Finn, Qibo Zhang
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?