Intestinal Microbial Ecology in Premature Infants Assessed with Non–Culture-Based Techniques - 07/03/14
, Jonathan Shuster, PhD c, d, Douglas Theriaque, MS d, Nan Li, MS, MD b, Volker Mai, MPH, PhD e
Reprint requests: Josef Neu, Department of Pediatrics, University of Florida, 1600 SW Archer Rd, Human Development Building Rm HD 513, Gainesville, FL 32610.Abstract |
Objectives |
To use high throughput techniques to analyze intestinal microbial ecology in premature neonates, who are highly susceptible to perturbations of the luminal environment associated with necrotizing enterocolitis (NEC) and late-onset sepsis.
Study design |
With non–culture-based techniques, we evaluated intestinal microbiota shortly after birth and during hospitalization in 23 neonates born at 23 to 32 weeks gestational age. Microbiota compositions were compared in 6 preterm infants in whom NEC, signs of systemic inflammation, or both developed with matched control subjects by using 16S ribosomal RNA pyrosequencing.
Results |
Microbial DNA was detected in meconium, suggesting an intrauterine origin. Differences in diversity were detected in infants whose mothers intended to breast feed (P = .03), babies born to mothers with chorioamnionitis (P = .06), and in babies born at <30 weeks gestation (P = .03). A 16S ribosomal RNA sequence analysis detected Citrobacter-like sequences only in cases with NEC (3 of 4) and an increased frequency of Enterococcus-like sequences in cases and Klebsiella in control subjects (P = .06). The overall microbiota profiles in cases with NEC were not distinguishable from that in control subjects.
Conclusions |
Microbial DNA in meconium of premature infants suggests prenatal influences.
Le texte complet de cet article est disponible en PDF.Mots-clés : DGGE, GI, NEC, OTU, PCR, rRNA, SDI
Plan
| Supported in part by grants from the National Institute of Research Resources, National Institutes of Health (RO1 HD 059143 and M01RR00082) and an educational grant (M.M.) from the European Society for Pediatric Research. The authors declare no conflicts of interest. |
Vol 156 - N° 1
P. 20-25 - janvier 2010 Retour au numéro
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