Domestic ruminants and Wild ungulates can act as reservoir hosts for Mycobacterium bovis, causative agent for bovine tuberculosis (BTB) in animals and man. Bovine tuberculosis (BTB) is endemic in pastoral livestock in Tanzania. There is lack of information on genotypic distribution of M. bovis in animals at wildlife-livestock interface areas and in wildlife conserved areas. The objectives of this study were to characterize and determine the spatial distribution of M. bovis isolates. Tissue samples from cattle and wildlife were cultured and analyzed using deletion typing and spoligotyping techniques. Eight (8.9%) M. bovis strains were isolated from cattle and confirmed by RD4 and RD9 polymerize chain reaction (PCR) assays. Spoligotype SB1467 was the dominant pattern 62.5% (n = 8) in infected animals, two isolates belonged to a newly reported type SB2190, and one isolate belonged to SB0133. The spoligotype patterns of SB1467 and SB0133 were closely related (96.9%) while SB2190 was less related (59.4%) to SB0133, the relatedness amongst spoligotypes were associated with spacer position 15. No M. bovis was isolated from hunted wild animals.

The current study has identified a novel spoligotype SB2190. The current data suggest that wild animals in Mikumi-Selous ecosystem are at risk of acquiring M. bovis infection due to occasional interaction by sharing of pasture and water sources between wildlife and livestock. Integrated efforts by all stakeholders are crucial for controlling spread of tuberculosis at livestock/wildlife/human interface areas.