pstS1 polymorphisms of Mycobacterium tuberculosis strains may reflect ongoing immune evasion - 15/08/13

Doi : 10.1016/j.tube.2013.05.003

Haican Liu ^a,^b,^{^{e
Haican Liu, Yi Jiang, Xiangfeng Dou and Haiyin Wang contributed equally to this study.}} , Yi Jiang ^a,^b,^{^{e
Haican Liu, Yi Jiang, Xiangfeng Dou and Haiyin Wang contributed equally to this study.}} , Xiangfeng Dou ^c,^{^{e
Haican Liu, Yi Jiang, Xiangfeng Dou and Haiyin Wang contributed equally to this study.}} , Haiyin Wang ^a,^b,^{^{e
Haican Liu, Yi Jiang, Xiangfeng Dou and Haiyin Wang contributed equally to this study.}} , Xiuqin Zhao ^a,^b , Wen Zhang ^a,^b , Li Wan ^a,^b , Zhijian Zhang ^d , Chen Chen ^a,^b,^⁎ , Kanglin Wan ^a,^b,^⁎
^a National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, Beijing 102206, PR China
^b Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, PR China
^c Beijing Center for Diseases Control and Prevention, Beijing 100013, PR China
^d Respiratory Diseases Department of Nanlou, Chinese People's Liberation Army General Hospital, 28# Fuxing Road, Haidian District, Beijing 100853, PR China

^∗Corresponding author. National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, P.O. Box 5, Changping, Beijing 102206, PR China. Tel./fax: +86 10 58900779.^∗∗Corresponding author. National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, P.O. Box 5, Changping, Beijing 102206, PR China. Tel.: +86 10 58900725.

Summary

The mycobacterial antigen PstS1 is a highly immunogenic and immunostimulatory component of the mycobacterial cell membrane and a good candidate for the diagnosis and vaccination against tuberculosis. Here we selected 180 clinical isolates of Mycobacterium tuberculosis complex (MTBC) in China and 11 different Bacille Calmette Guerin (BCG) strains, amplified the gene of the PstS1 antigen and compared the sequences with those of four other Mycobacterium bovis and BCG strains from the NCBI genome website. Some of the mutations, especially 2 frameshift mutations, occurred in the PstS1antigen, which may have resulted in the protein function alteration and ongoing immune evasion. A unique single nucleotide polymorphism of the M. bovis and BCG strains was found in this antigen and may be useful for differentiating M. bovis and BCG strains from M. tuberculosis strains.

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Keywords : PstS1 polymorphisms, Mycobacterium tuberculosis complex, Immune evasion

Plan

Materials and methods

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Strains and DNA preparation

Mutation and insertion

Changes in protein level

Changes in the T cell epitope of PstS1

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Vol 93 - N° 5

P. 475-481 - septembre 2013 Retour au numéro

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pstS1 polymorphisms of Mycobacterium tuberculosis strains may reflect ongoing immune evasion - 15/08/13

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