Stimulated Nitric Oxide Production and Arginine Deficiency in Children with Cystic Fibrosis with Nutritional Failure - 23/07/13
, Gulnur Com, MD 2, Yvette C. Luiking, PhD 1, Nicolaas E.P. Deutz, MD, PhD 1, 3Abstract |
Objective |
To determine whether upregulated whole body de novo arginine synthesis and protein breakdown are present as a compensatory mechanism to meet the increased demand for arginine and nitric oxide (NO) production in pediatric patients with cystic fibrosis (CF) and nutritional failure.
Study design |
In 16 children with CF, studied at the end of antibiotic treatment for a pulmonary exacerbation, and 17 healthy controls, whole body arginine, citrulline (Cit), and protein turnover were assessed by stable isotope methodology and de novo arginine synthesis, arginine clearance, NO synthesis, protein synthesis and breakdown, and net protein balance were calculated. The plasma isotopic enrichments and amino acid concentrations were measured by liquid chromatography–tandem mass spectrometry.
Results |
Increased arginine clearance was found in patients with CF (P < .001), whereas whole body NO production rate and plasma arginine levels were not different. Whole body arginine production (P < .001), de novo arginine synthesis, and protein breakdown and synthesis (P < .05) were increased in patients with CF, but net protein balance was comparable. Patients with CF with nutritional failure (n = 7) had significantly higher NO production (P < .05), de novo arginine synthesis, Cit production (P < .001), and plasma Cit concentration (P < .05) and lower plasma arginine concentration (P < .05) than those without nutritional failure (n = 9).
Conclusions |
Nutritional failure in CF is associated with increased NO production. However, up-regulation of de novo arginine synthesis and Cit production was not sufficient to meet the increased arginine needs leading to arginine deficiency.
Le texte complet de cet article est disponible en PDF.Keyword : ADMA, Arg, CF, Cit, FeNO, FEV1, FFM, FM, NO, Orn, WbRa
Plan
| Supported by the Arkansas Children’s Hospital Research Institute and the Arkansas Biosciences Institute (major research component of the Tobacco Settlement Proceeds Act of 2000), a partnership of scientists from Arkansas Children’s Hospital, Arkansas State University, the University of Arkansas for Medical Sciences, the University of Arkansas Division of Agriculture, the University of Arkansas, Fayetteville, and the National Institutes of Health (S10RR027047). Y. L. is an employee of Nutricia Advanced Medical Nutrition, Danone Research–Centre for Specialized Nutrition, Wageningen, The Netherlands. The authors declare no conflicts of interest. |
Vol 163 - N° 2
P. 369 - août 2013 Retour au numéro
Article précédent
- Childhood Alcohol Use May Predict Adolescent Binge Drinking: A Multivariate Analysis among Adolescents in Brazil
- Zila M. Sanchez, Mariana G.R. Santos, Ana Paula D. Pereira, Solange A. Nappo, Elisaldo A. Carlini, Claudia M. Carlini, Silvia S. Martins
- Pubertal Height Velocity and Associations with Prepubertal and Adult Heights in Cystic Fibrosis
- Zhumin Zhang, Mary J. Lindstrom, HuiChuan J. Lai
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?