Proinflammatory role of epithelial cell–derived exosomes in allergic airway inflammation - 30/03/13
![](/templates/common/images/mail.png)
![](/templates/common/images/entites/lowast.gif)
Abstract |
Background |
Exosomes are nanovesicles involved in intercellular communication. Their roles in various diseases are often contextual, depending on the cell type producing them. Although few studies hint toward the proinflammatory role of bronchoalveolar lavage fluid–derived exosomes in asthmatic progression, the cell types in lungs associated with exosome-mediated crosstalk and their resultant effects remain unexplored.
Objective |
It is well established that exosome-mediated cellular communication can influence disease phenotypes. This study explores exosome-mediated cellular crosstalk between structural and immune cells in asthma pathogenesis.
Methods |
Exosomes were isolated and detected from bronchoalveolar lavage fluid of control and asthmatic mice and were quantified by using a bead-based assay. Involvement of epithelial cells and macrophages were established by using immunohistochemical techniques in lung tissue sections. The role of IL-13 in exosome production was ascertained by using various in vitro and in vivo techniques. Exosome secretion was blocked in in vitro and in vivo settings by using a chemical inhibitor, and the effects on various asthmatic features were studied.
Results |
Using combinatorial in vitro and in vivo approaches, we found that exosome secretion and production of exosome-associated proteins are higher in lungs of asthmatic mice compared with that seen in sham mice. Asthma is marked by enhanced secretion of exosomes by epithelial cells, but not macrophages, under the influence of IL-13. These epithelial cell exosomes induce proliferation and chemotaxis of undifferentiated macrophages. On the other hand, GW4869, which inhibited exosome production, resulted in a reduced population of proliferating monocytes and alleviation of various asthmatic features.
Conclusion |
Under the influence of IL-13, epithelial cell–derived exosomes can induce enhanced proliferation and chemotaxis of undifferentiated macrophages in the lungs during asthmatic inflammatory conditions.
Le texte complet de cet article est disponible en PDF.Key words : Asthma, allergy, exosomes, epithelial cells, IL-13, monocytes
Abbreviations used : AAI, AHR, APC, BALF, FITC, Hsp-70, OVA
Plan
Supported by grants from the Council of Scientific and Industrial Research (CSIR), India (Task Force Project NWP0033, MLP5501). |
|
Disclosure of potential conflict of interest: A. Kulshreshtha has received grants from the Council of Scientific and Industrial Research and has received travel support from the Department of Science and Technology, Government of India. A. Agrawal has received grants from the Council of Scientific and Industrial Research. The rest of the authors declare that they have no relevant conflicts of interest. |
Vol 131 - N° 4
P. 1194 - avril 2013 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?