The effect of ghrelin on cell proliferation in small intestinal IEC-6 cells - 25/03/13
Corresponding author. Department of Gastroenterology, Jinshan Hospital of Fudan University, No. 1508, Longhang Road, Jinshan, Shanghai 201508, China. Tel.: +86 21 341 899 90 5389; fax: +86 21 672 26910.Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
| pages | 5 |
| Iconographies | 4 |
| Vidéos | 0 |
| Autres | 0 |
Abstract |
Background and aims |
Recent evidence demonstrates that ghrelin, a short orexigenic peptide from the stomach, has dual effects on cell proliferation in different cell types via autocrine and/or paracrine mechanisms. The aim of this study is to investigate the proliferative role of ghrelin in intestinal epithelial IEC-6 cells and explore underlying mechanism.
Methods |
RT-PCR was used for the detection of growth hormone secretagogue receptor 1a. Cell proliferation was measured using Cell Counting Kit-8. Protein expression of ERK 1/2 and Akt was examined using western blotting. Inhibitors of mitogen activated protein kinases kinase and phosphatidylinositol 3-kinase were used to evaluate the role of these signalling pathways in ghrelin-induced proliferation of IEC-6 cells.
Results |
Growth hormone secretagogue receptor 1a mRNA was present in IEC-6 cells. Ghrelin and des-acyl ghrelin increased IEC-6 cell proliferation in a dose- and time-dependent manner. Ghrelin and des-acyl ghrelin activated ERK1/2, but not Akt. U0126, a specific inhibitor of mitogen activated protein kinases kinase, blocked ghrelin- and des-acyl ghrelin-induced ERK1/2 phosphorylation and cell proliferation in IEC-6 cells.
Conclusion |
Ghrelin and des-acyl ghrelin stimulate the proliferation of IEC-6 cells via the ERK1/2 pathway.
Le texte complet de cet article est disponible en PDF.Keywords : Ghrelin, Cell proliferation, ERK1/2, IEC-6
Plan
Vol 67 - N° 3
P. 235-239 - avril 2013 Retour au numéro
Article précédent
- Overexpression of JAKMIP1 associates with Wnt/beta-catenin pathway activation and promotes cancer cell proliferation in vitro
- Isaac Okai, Lan Wang, Linlin Gong, Benjamin Arko-Boham, Lihong Hao, Xin Zhou, Xiaoyu Qi, Jun Hu, Shujuan Shao
- Microarray-based detection and expression analysis of ABC and SLC transporters in drug-resistant ovarian cancer cell lines
- Radosław Januchowski, Piotr Zawierucha, Małgorzata Andrzejewska, Marcin Ruciński, Maciej Zabel
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’achat d’article à l’unité est indisponible à l’heure actuelle.
Déjà abonné à cette revue ?