Mast cell anaphylatoxin receptor expression can enhance IgE-dependent skin inflammation in mice - 30/01/13
, Adrian M. Piliponsky, PhD a, b, , Tatsuya Oka, PhD a, Chang Ho Song, MD, PhD a, c, Norma P. Gerard, PhD d, Craig Gerard, MD, PhD d, Mindy Tsai, DMSc a, Janet Kalesnikoff, PhD a, Stephen J. Galli, MD a, e, ⁎ 
Corresponding author: Stephen J. Galli, MD, Department of Pathology, Lane Building, L-235, Stanford University School of Medicine, 300 Pasteur Dr, Stanford, CA 94305-5324.Abstract |
Background |
Mast cells express receptors for complement anaphylatoxins C3a and C5a (ie, C3a receptor [C3aR] and C5a receptor [C5aR]), and C3a and C5a are generated during various IgE-dependent immediate hypersensitivity reactions in vivo. However, it is not clear to what extent mast cell expression of C3aR or C5aR influences C3a- or C5a-induced cutaneous responses or IgE-dependent mast cell activation and passive cutaneous anaphylaxis (PCA) in vivo.
Objective |
We sought to assess whether mouse skin mast cell expression of C3aR or C5aR influences (1) the cells’ responsiveness to intradermal injections of C3a or C5a or (2) the extent of IgE-dependent mast cell degranulation and PCA in vivo.
Methods |
We measured the magnitude of cutaneous responses to intradermal injections of C3a or C5a and the extent of IgE-dependent mast cell degranulation and PCA responses in mice containing mast cells that did or did not express C3aR or C5aR.
Results |
The majority of the skin swelling induced by means of intradermal injection of C3a or C5a required that mast cells at the site expressed C3aR or C5aR, respectively, and the extent of IgE-dependent degranulation of skin mast cells and IgE-dependent PCA was significantly reduced when mast cells lacked either C3aR or C5aR. IgE-dependent PCA responses associated with local increases in C3a levels occurred in antibody-deficient mice but not in mice deficient in Fc
RIγ.
Conclusion |
Expression of C3aR and C5aR by skin mast cells contributes importantly to the ability of C3a and C5a to induce skin swelling and can enhance mast cell degranulation and inflammation during IgE-dependent PCA in vivo.
Le texte complet de cet article est disponible en PDF.Key words : Anaphylatoxin, anaphylaxis, C3a, C5a, complement, IgE, inflammation, mast cells
Abbreviations used : BMCMC, C3aR, C5aR, DNP, HSA, PCA, PCMC, WT
Plan
| Supported by National Institutes of Health grants AI023990, CA072074, and AI070813 (to S.J.G.) and a grant of the Deutsche Forschungsgemeinschaft (Scha1464/1-1 to B.S.). |
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| Disclosure of potential conflict of interest: S. J. Galli has received research support from the National Institutes of Health and the Stanford University Department of Pathology. The rest of the authors declare that they have no relevant conflicts of interest. |
Vol 131 - N° 2
P. 541 - février 2013 Retour au numéro
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