Interferon-β induces a long-lasting antiviral state in human respiratory epithelial cells - 04/01/13
Corresponding author. Department of Medical Microbiology, Maastricht University Medical Center, PO Box 5800, 6202 AZ Maastricht, The Netherlands. Tel.: +31 43 387 6644; fax: +31 43 387 6643.Summary |
Objectives |
Interferon-β (IFNβ) induces strong antiviral effects and is therefore an attractive agent to prevent or reduce the incidence of virus-mediated exacerbations in asthmatic or chronic obstructive pulmonary disease (COPD) patients. We therefore investigated the effects of prophylactic IFNβ on respiratory epithelial cells infected with rhinovirus (RV).
Methods |
A549 cells and primary bronchial epithelial cells (PBECs) were exposed for 18 h to IFNβ. Then, IFNβ was either removed or maintained in the supernatant for the rest of the experiment and cells were infected with RV-1B at t = 0 or 72 h after the initial exposure to IFNβ.
Results |
Viral RNA levels were decreased in both cell types. Furthermore, both viral RNA and infectious virus levels in the supernatant of infected A549 cells were still significantly reduced at 72 h after removal of IFNβ. This pronounced antiviral pre-treatment effect was associated with increased expression of the antiviral genes IFN-stimulated protein of MR15000 (ISG15) and Myxovirus resistance 1 (Mx1) and the effect was maintained even when IFNβ levels in the supernatant of A549 cells were undetectable.
Conclusions |
These data show that IFNβ has not only a strong, but also a long-lasting protective effect against RV infection of respiratory epithelium.
Le texte complet de cet article est disponible en PDF.Keywords : Interferon β, Rhinovirus, A549 cells, Primary bronchial epithelial cells, Asthma, COPD
Plan
Vol 66 - N° 2
P. 163-169 - février 2013 Retour au numéro
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