Proliferation and differentiation of osteoblastic cells on silicon-doped TiO2 film deposited by cathodic arc - 22/11/12
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Abstract |
This study aimed at the proliferation and differentiation of osteoblastic cells on silicon-doped TiO2 and pure TiO2 films prepared by cathodic arc deposition. The films were examined by X-ray photo-electron spectroscopy, which showed that silicon was successfully doped into the Si-TiO2 film. Meanwhile, no significant difference was found between the surface morphology of silicon-doped TiO2 and pure TiO2 films. When osteoblastic cells were cultured on silicon-doped TiO2 film, accelerated cell proliferation was observed. Furthermore, cell differentiation was evaluated using alkaline phosphatase (ALP), type I collagen (COL I) and osteocalcin (OC) as differentiation markers. It was found that ALP activity, the expression levels of OC gene, COL I gene and protein were up-regulated on silicon-doped TiO2 film at 3 and 5 days of culture. Moreover, no significant difference was found in apoptosis between the cells cultured on silicon-doped TiO2 and pure TiO2 films. Therefore, findings from this study indicate that silicon-doped film favors osteoblastic proliferation and differentiation, and has the potential for surface modification of implants in the future.
Le texte complet de cet article est disponible en PDF.Keywords : Osteoblast, Silicon, TiO2 film, Cathodic arc deposition
Plan
Vol 66 - N° 8
P. 633-641 - décembre 2012 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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