D2-40 immunohistochemical overexpression in cutaneous squamous cell carcinomas: A marker of metastatic risk - 14/11/12
, Javier Gimeno-Beltrán, MD b, Carla Ferrandiz-Pulido, MD c, Emili Masferrer, MD a, Mireia Yébenes, MD d, Anna Jucglà, MD f, Leandre Abal, MD g, Rosa M. Martí, MD g, Onofre Sanmartín, MD h, Teresa Baró, PhD b, Beatriz Casado, BMedSci b, Alberto Gandarillas, PhD i, Carlos Barranco, MD b, Irmgard Costa, MD e, Sergi Mojal, BMS j, Vicenç García-Patos, MD c, Ramon M. Pujol, MD a
Reprint requests: Agustí Toll, MD, Department of Dermatology, Hospital del Mar, Parc de Salut Mar, Pg/Marítim 25-29, 08003-Barcelona, Spain.Abstract |
Background |
Approximately 4% of cutaneous squamous cell carcinomas (cSCCs) develop lymphatic metastases. The value of lymphatic endothelial markers to enhance the detection of lymphatic tumor invasion in cSCC has not been assessed previously.
Objective |
We sought to evaluate the use of the antibody D2-40, a podoplanin immunohistochemical marker, to identify tumor lymph vessel invasion in cSCC and to assess its expression in tumor cells.
Methods |
This was a retrospective case-control study. A series of 101 cSCC, including 51 cases that developed lymphatic metastatic spread (metastasizing cSCC [MSCC]) and 50 cases that resolved definitely after surgical excision (non-MSCC) were included in the study. Lymph vessel invasion using D2-40 was evaluated on all primary biopsy specimens. The percentage of tumor cells showing D2-40 positivity and intensity scoring were recorded. All the immunohistochemical findings were correlated with the clinicopathological features.
Results |
Lymph vessel invasion was observed in 8% of non-MSCCs and in 25.5% of MSCCs (P = .031). D2-40 expression was significantly increased, both in intensity (odds ratio 4.42 for intensity ++/+++) and in area (odds ratio 2.29 for area >10%), in MSCC when compared with non-MSCC. Interestingly, almost half (49%) of the MSCC had moderate to intense D2-40 positivity compared with 16% of non-MSCC. D2-40 immunohistochemical expression was increased in tumors with an infiltrative pattern of extension. In the multivariate analysis, histologically poorly differentiated tumors, recurrent lesions, and cSCC showing D2-40 overexpression (in intensity) were significantly associated with lymphatic metastases development (odds ratios 15.67, 14.72, and 6.07, respectively).
Limitations |
This was a retrospective study.
Conclusion |
The expression of podoplanin associates with high metastatic risk in cSCC.
Le texte complet de cet article est disponible en PDF.Key words : D2-40, lymph node, lymphatic invasion, metastasizing, podoplanin, squamous cell carcinoma
Abbreviations used : AJCC, CI, cSCC, LVI, MSCC, SCC
Plan
| Supported in part by grants PI041728, PI10/00785, and RD09/0076/00036 from Fondo de Investigación Sanitaria, Instituto de Salud Carlos III, Ministerio de Sanidad, Federación Española de Enfermedades Raras (FEDER), Spain, and the Xarxa de Bancs de tumors sponsored by Pla Director d’Oncologia de Catalunya. |
|
| Conflicts of interest: None declared. |
Vol 67 - N° 6
P. 1310-1318 - décembre 2012 Retour au numéro
Article précédent
- The safety of Mohs surgery: A prospective multicenter cohort study
- Bradley G. Merritt, Nicole Y. Lee, David G. Brodland, John A. Zitelli, Joel Cook
- Giant angiofibromas in tuberous sclerosis complex: A possible role for localized lymphedema in their pathogenesis
- Denisa Kacerovska, Katrin Kerl, Michal Michal, Hana Filipova, Radek Vrtel, Tomas Vanecek, Hana Zelenakova, Jaroslav Kraus, Roman Kodet, Dmitry V. Kazakov
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?