Effect of Angiotensin II Receptor Antagonist Telmisartan on Detrusor Overactivity in Rats With Bladder Outlet Obstruction - 30/10/12
Reprint requests: Joon Chul Kim, M.D., Ph.D., Department of Urology, Bucheon St. Mary's Hospital, College of Medicine, the Catholic University of Korea, 2, Sosa-dong, Wonmi-gu, Bucheon City, Gyeonggi-do, 420-717, KoreaRésumé |
Objective |
To investigate the changes of expression in both the nerve growth factor (NGF) and the angiotensin II type 1 (AT1) receptors in the urothelium and the detrusor muscle in a rat model of bladder outlet obstruction (BOO). To determine whether the AT1 receptor antagonist telmisartan alleviated the detrusor overactivity in rats with BOO.
Methods |
Male Sprague–Dawley rats were randomly assigned to 3 groups. The control group (n = 10) was sham-operated. The animals in the BOO (n = 20) and the telmisartan groups (n = 20) underwent a partial BOO operation. The telmisartan group received telmisartan (3 mg/kg/d) for 14 days. Cystometry was performed in all 3 groups for 2 weeks after surgery. The expression levels and the cellular distribution of NGF and AT1 receptors were quantified by Western blot analysis and immunofluorescence staining.
Results |
On cystometry, the intercontraction interval was shorter and the mean number of nonvoiding contractions was increased in the BOO group, compared with those in the control group. The intercontraction interval was longer and the number of nonvoiding contractions was reduced in the telmisartan group, compared with those in the BOO group. In Western blotting and immunofluorescence staining, we found that the expression and the immunoreactivity of NGF and the AT1 receptors were increased in the BOO group, compared with those in the control group. However, with oral administration of telmisartan, those of NGF and AT1 were decreased.
Conclusion |
The AT1 receptors may play a significant role in the pathogenesis of the detrusor overactivity in a rat model of BOO.
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| Financial Disclosure: The authors declare that they have no relevant financial interests. |
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| Funding Support: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology (2010-0006069). |
Vol 80 - N° 5
P. 1163.e1-1163.e7 - novembre 2012 Retour au numéro
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