Identical MALDI TOF MS-derived peak profiles in a pair of isogenic SCCmec-harboring and SCCmec-lacking strains of Staphylococcus aureus - 09/10/12
, Martin Kaase, Agnes Anders, Sören G. Gatermann
Corresponding author. Tel.: +49 0 234 32 26467; fax: +49 0 234 32 14197.Summary |
MALDI-TOF MS-based peak differences in oxacillin-resistant Staphylococcus aureus and oxacillin-susceptible S. aureus isolates have been described previously. Unfortunately, these isolates were not isogenic with respect to their mecA gene. Ours is the first to use a SCCmec-harboring parent and a SCCmec-lacking daughter strain, with the same genetic background, to unequivocally rule out strain-specific protein peaks. We could not show differences in the peak profiles within the preset Biotyper settings used for MALDI-TOF-based identification in this pair of SCCmec-harboring parent and SCCmec-lacking daughter strains.
Le texte complet de cet article est disponible en PDF.Keywords : Staphylococcus aureus, MALDI-TOF MS, Isogenic, SCCmec, mecA
Plan
Vol 65 - N° 5
P. 400-405 - novembre 2012 Retour au numéro
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