Gene expression profiling reveals the defining features of monocytes from septic patients with compensatory anti-inflammatory response syndrome - 09/10/12
, Xiao-ming Deng b, ⁎ 
Corresponding author. Tel./fax: +86 21 64175590.Corresponding author. Tel./fax: +86 21 81873484.Summary |
Objectives |
To characterize the expression profiles of genes in purified monocytes from septic patients during systemic inflammatory response syndrome (SIRS) and compensatory anti-inflammatory response syndrome (CARS), and then to investigate the potential mechanism of monocyte deactivation.
Methods |
Lipopolysaccharides (LPS)-induced cytokine responses, phagocytosis assay and migration assay were performed in monocytes from SIRS patients, CARS patients and healthy volunteers (n = 8). After functional assays, each pair of samples from the same group was pooled into one for gene expression analysis. All new samples (n = 4) were hybridized on NimbleGen human gene expression 12 × 135 K microarrays, and selected genes were validated by real-time polymerase chain reaction. Pathway analysis and Gene Ontology analysis were performed on differentially expressed genes using Agilent GeneSpring (version 11.0).
Results |
A set of genes related to pro-inflammation, phagocytosis, chemotaxis, antigen presentation, and anti-apoptosis were significantly down-regulated, while some genes associated with pro-apoptosis and anti-inflammation were up-regulated instead on monocytes from CARS patients compared with SIRS patients and healthy volunteers. Monocytes from CARS patients showed impaired production of TNF-⍺ and IL-6, and increased release of IL-10 when stimulated by LPS. Functional analysis confirmed reduced phagocytosis and migratory activity of monocytes from CARS patients. Human leukocyte antigen-DR (HLA-DR) measurements demonstrated decreased expression of HLA-DR on monocytes from CARS patients.
Conclusion |
Monocytes from CARS patients exhibited significant changes in mRNA expression of genes associated with phagocytosis, antigen presentation, inflammatory response, cell migration, and apoptosis, which might cause deactivation of monocytes during CARS.
Le texte complet de cet article est disponible en PDF.Keywords : Sepsis, Septic shock, Systemic inflammatory response syndrome, Compensatory anti-inflammatory response syndrome, Monocyte deactivation
Plan
Vol 65 - N° 5
P. 380-391 - novembre 2012 Retour au numéro
Article précédent
- European Antibiotic Awareness Day 2012: Getting smart about antibiotics, a public–professional partnership
- J.M. Stockley
- Bacteremia caused by antimicrobial resistant Campylobacter species at a medical center in Taiwan, 1998–2008
- Chun-Hsing Liao, Chia-Yunn Chuang, Yu-Tsung Huang, Ping-Ing Lee, Po-Ren Hsueh
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?