Increased interleukin-32 expression in chronic hepatitis B virus-infected liver - 09/09/12

Doi : 10.1016/j.jinf.2012.05.009

Qihuan Xu ^a,^{^{g
Qihuan Xu and Xingfei Pan contributed equally to this work.}}, Xingfei Pan ^a,^{^{g
Qihuan Xu and Xingfei Pan contributed equally to this work.}}, Xin Shu ^a, Hong Cao ^a, Xuejun Li ^a, Ka Zhang ^a, Jianxi Lu ^b, Yong Zou ^c, Xueling Li ^d, Hongcan Liu ^b, Yeqiong Zhang ^a, Daofeng Yang ^e, Qin Ning ^e, Guanxin Shen ^f, Gang Li ^a,^⁎
^a Department of Infectious Diseases, The 3rd Affiliated Hospital, Sun Yat-Sen University, No.600, Tianhe Road, Guangzhou 510630, China
^b Vaccine Research Institute, The 3rd Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510630, China
^c Department of Blood Transfusion, The 3rd Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510630, China
^d Department of Biological Engineering, Food College of South China Agricultural University, Guangzhou 510640, China
^e Department of Infectious Diseases, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, China
^f Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

Corresponding author. Tel.: +86 20 85253195.

Summary

objectives

The role of interleukin-32 (IL-32) in chronic hepatitis B (CHB) remains unclear. In order to identify the role of IL-32 in CHB, we detected the expression levels of IL-32 in liver samples of CHB patients and analyzed the correlation between IL-32 and liver inflammation/fibrosis.

Methods

Real-time PCR and immunohistochemistry were used to detect the expression levels of IL-32 in liver tissues of patients with CHB. The correlation between hepatic IL-32 expression and the severity of liver inflammation/fibrosis was assessed using Spearman’s correlation.

Results

Hepatic IL-32 expression was increased in CHB patients and increased with the severity of liver inflammation/fibrosis. Moreover, hepatic IL-32 expression was significantly positively correlated with serum ALT level and negatively related with serum ALB level.

Conclusions

Our results suggest that IL-32 could be implicated in HBV-related liver inflammation/fibrosis. We believe that IL-32 might play important roles in the pathogenesis of CHB.

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Keywords : Chronic hepatitis B, IL-32, Inflammation

Plan

Introduction

Materials and methods

Subjects

Sample collection and liver histology assessment

Immunohistochemistry

Image analysis

Detection of IL-32 mRNA expression levels

Statistics analysis

Results

IL-32 mRNA expressions were increased in liver tissues of CHB patients

IL-32 protein expressions were augmented in hepatic tissues of CHB patients

Hepatic IL-32 expression was positively related with serum ALT level

Hepatic IL-32 expression was negatively correlated with serum ALB level

Discussion

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Vol 65 - N° 4

P. 336-342 - octobre 2012 Retour au numéro

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Increased interleukin-32 expression in chronic hepatitis B virus-infected liver - 09/09/12

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