Effects of Left Ventricular Assist Device (LVAD) Placement on Myocardial Oxidative Stress Markers - 27/07/12
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Résumé |
Purpose |
The primary purpose of this study was to examine the changes in myocardial oxidative stress during the support of a left ventricular assist device (LVAD).
Methods |
Myocardial tissue was collected from the lower left ventricle of 15 adult subjects with class IV heart failure (HF) during LVAD placement (n=9) or LVAD removal (Post-LVAD; n=6). Each tissue sample was separated into cytosolic and myofibrillar subfractions and analysed for protein content and carbonylation.
Results |
The myofibrillar proteins in the HF subjects had a significantly lower (p=0.008) level of protein carbonylation when compared to the myofibrillar proteins in Post-LVAD patients at 1.630±0.277 and 3.075±0.413 optical density, respectively. The level of protein carbonylation in myosin and actin were lower in HF (myosin: 1406.22±218.45, actin: 436±79.72 optical density) subjects compared to Post-LVAD (myosin: 2280.5±441.26, actin: 804.67±155.71 optical density) subjects (p=0.035 and p=0.018, respectively). However, once the extent of carbonylation in the myosin and actin bands were normalised to the amount of protein content, all significant difference was lost (HF moysin: 1823.89±413.42, Post-LVAD myosin: 1330.33±297.10 optical density, p=0.199 and HF actin: 3755.78±349.59, Post-LVAD actin: 4402.83±666.51 optical density, p=0.182). There was no significant difference in the cytosolic subfractions before or after normalisation of protein content.
Conclusion |
Carbonylation is elevated in the myocardium of HF and Post-LVAD subjects and it appears that LVAD support does not affect the level of myocardial oxidative stress.
Le texte complet de cet article est disponible en PDF.Abbreviations : BCA, BMI, HF, HNE, LVAD, NT
Keywords : Heart failure, Left ventricular assist device, Myocardium, Oxidative stress
Plan
Vol 21 - N° 9
P. 586-597 - septembre 2012 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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