Rapid diagnosis of influenza: An evaluation of two commercially available RT-PCR assays - 25/05/12
, Nigel Brenwald a, b , Sandra Bonigal a, b , Kulvir Chana a, b , Husam Osman a, b , Beryl Oppenheim a, b 
Corresponding author. Department of Microbiology, City Hospital, Dudley Road, Birmingham, B18 7QH, United Kingdom. Tel.: +44 0121 507 4078; fax: +44 0121 507 5521.Summary |
Objective |
To evaluate the sensitivity and specificity of influenza virus detection by two commercial reverse transcriptase PCR methods compared with a reference real-time PCR.
Methods |
122 clinical specimens were tested on Xpert® Flu and RealStar® Influenza Screen & Type. A reference real-time RT-PCR, at a specialist laboratory was chosen as the gold standard for comparison.
Results |
RealStar® Influenza Screen & Type had higher sensitivity for influenza A and influenza B respectively (92.3% and 88.2%) when compared to Xpert® Flu (78.8% and 76.5%). Both tests had excellent specificity.
Conclusions |
The simplicity and speed of the Xpert® Flu system could allow it to be used in the near-patient setting; however in circumstances where excluding a diagnosis of influenza may be critical, negative specimens may need to be repeated using a more sensitive assay.
Le texte complet de cet article est disponible en PDF.Keywords : Influenza, Commercial, PCR, Comparison
Plan
Vol 65 - N° 1
P. 60-63 - juillet 2012 Retour au numéro
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