Expression of innate defense antimicrobial peptides in hidradenitis suppurativa - 13/05/12
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Abstract |
Background |
Hidradenitis suppurativa (HS) is a chronic inflammatory disorder of apocrine gland-bearing skin. It is associated with alterations in innate immunity and frequent bacterial infections.
Objective |
We investigated the expression of innate defense antimicrobial peptides in patients with HS and different grades of severity.
Methods |
Skin biopsy specimens and sweat were collected from 36 patients with HS and 57 healthy control subjects for analysis of epithelial antimicrobial peptides by real-time reverse transcription–polymerase chain reaction, immunohistochemistry, enzyme-linked immunosorbent assay, and surface-enhanced laser desorption ionization time-of-flight mass spectrometry.
Results |
We provide evidence that human β-defensin-3 expression is induced in lesional HS skin on transcriptional and protein levels, yet, this up-regulation was not detectable in patients with severe HS (Hurley grade III). In contrast, messenger RNA expression of ribonuclease 7 was significantly diminished in lesional HS skin specimens irrespective of HS severity. Overall levels of dermcidin/dermcidin-derived peptides in sweat and messenger RNA expression of psoriasin in skin biopsy specimens did not differ between patients with HS and healthy control subjects.
Limitation |
The relatively small number of samples, in particular in the group of patients with HS and Hurley grade III, is a limitation.
Conclusion |
Deficient constitutive production of ribonuclease 7 and, in severe HS, reduced human β-defensin-3 induction may contribute to impaired immunity within the hair follicle and thereby boost HS inflammation and severity.
Le texte complet de cet article est disponible en PDF.Key words : acne inversa, antimicrobial peptides, hidradenitis suppurativa, human β-defensin-3, innate immunity, ribonuclease 7, Staphylococcus aureus
Abbreviations used : AMP, ELISA, HBD, HS, IL, mRNA, PCR, RNase, SELDI-TOF-MS
Plan
Supported by grant 01EO0803 from the German Ministry of Education and Research to the Center for Chronic Immunodeficiency, University Freiburg–Medical Center. |
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Conflicts of interest: None declared. |
Vol 66 - N° 6
P. 966-974 - juin 2012 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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