Characterization of Hydrogen Sulfide and Its Synthases, Cystathionine β-Synthase and Cystathionine γ-Lyase, in Human Prostatic Tissue and Cells - 06/02/12
Reprint requests: Jie Jin, M.D., Department of Urology, Peking University First Hospital, Institute of Urology, Peking University, Beijing 100034 ChinaRésumé |
Objective |
To investigate hydrogen sulfide and its synthases, cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE), in human prostatic tissue and cells.
Methods |
CBS and CSE in human prostatic tissue and cells were located using immunostaining. Western blot and a sulfur-sensitive electrode were used to evaluate the expression levels and catalytic activity of CBS and CSE. We analyzed the association between dihydrotestosterone-added or hormone-reduced medium-induced CBS/CSE protein levels with androgen receptor levels in prostate cancer lines. All experiments were repeated ≥3 times.
Results |
Endogenous hydrogen sulfide and its synthases existed in various areas of human prostatic tissue and cells. Cell activity and CBS/CSE protein levels were greatest in the androgen-dependent prostate cancer cell LNCaP among all cells and downregulated by dihydrotestosterone.
Conclusion |
Hydrogen sulfide and its synthases in human prostatic tissue and cells were modulated by dihydrotestosterone, which could suggest a potential therapy for prostatic disease.
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| Funding Support: This work was supported by grants 30571851 from the National Natural Science Foundation of China (to J. Jin). |
Vol 79 - N° 2
P. 483.e1-483.e5 - février 2012 Retour au numéro
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