Airway TGF-β1 and oxidant stress in children with severe asthma: Association with airflow limitation - 03/02/12
Corresponding author: Anne M. Fitzpatrick, PhD, MSCR, 2015 Uppergate Dr, Atlanta, GA, 30322.Abstract |
Background |
TGF-β1 is thought to play a role in airway remodeling in asthmatic subjects. TGF-β1 expression might be mediated by an excessive burden of reactive oxygen species and oxidant stress.
Objective |
Given the profound airway oxidant stress we have previously observed in children with severe asthma, we sought to (1) quantify TGF-β1 protein and mRNA gene expression in the airways of children with mild-to-moderate and severe atopic asthma and (2) determine the relationship of airway TGF-β1 concentrations to oxidant burden (ie, lipid peroxidation), TH2-mediated eosinophilic inflammation, and airflow limitation.
Methods |
Bronchoalveolar lavage fluid was collected from 68 atopic children with asthma (severe asthma, n = 28) and 12 atopic adult control subjects. Airway TGF-β1 expression and activation were assessed in relation to airway IL-13, 8-isoprostane, and malondialdehyde concentrations. The relationship of airway TGF-β1 expression to airflow limitation in children with asthma was also assessed.
Results |
Children with severe asthma had higher total airway concentrations of TGF-β1 that were associated with increased protein and mRNA expression of TGF-β1 in airway macrophages and an increase in concentrations of the lipid peroxidation biomarkers 8-isoprostanes and malondialdehyde. TGF-β1 activation was also greater in children with severe asthma and was associated with higher airway 8-isoprostane, malondialdehyde, and IL-13 concentrations. Total airway TGF-β1 concentrations were further associated with airflow limitation.
Conclusions |
Children with severe asthma have increased airway TGF-β1 expression and activation associated with an increased airway oxidant burden. Oxidant stress might mediate the effects of TGF-β1 and promote airway remodeling in children with severe asthma.
Le texte complet de cet article est disponible en PDF.Key words : Airway remodeling, asthma, children, lung function, oxidant stress, TGF-β1
Abbreviations used : BAL, Ct, ELF, ICS, Nrf2, OR
Plan
| Supported by National Institutes of Health RO1 NR012021 and supported in part by PHS Grant UL1RR025008 from the Clinical and Translational Science Award program, National Institutes of Health, National Center for Research Resources. |
|
| Disclosure of potential conflict of interest: A. M. Fitzpatrick receives research support from the National Institutes of Health/National Institute of Nursing Research and National Center for Research Resources. The rest of the authors declare that they have no relevant conflicts of interest. |
Vol 129 - N° 2
P. 388 - février 2012 Retour au numéro
Article précédent
- The role of the small airways in the clinical expression of asthma in adults
- Claude S. Farah, Gregory G. King, Nathan J. Brown, Sue R. Downie, Jessica A. Kermode, Kate M. Hardaker, Matthew J. Peters, Norbert Berend, Cheryl M. Salome
- Addendum
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?