Humoral and Cell-Mediated Immune Responses to Monovalent 2009 Influenza A/H1N1 and Seasonal Trivalent Influenza Vaccines in High-Risk Children - 08/12/11
, 1, Ana Fernandez-Sesma, PhD , 6, Rebecca Pellett Madan, MD, MSc 1, ⁎ 
Reprint requests: Rebecca Pellett Madan, MD, MSc, Albert Einstein College of Medicine, Department of Pediatrics, Forchheimer 702D, 1300 Morris Park Ave, Bronx, NY 10461.Abstract |
Objective |
Humoral and cell-mediated immune responses to monovalent 2009 pandemic influenza A (H1N1/2009) and seasonal trivalent influenza (TIV) vaccines were evaluated in healthy children and children with asthma, sickle cell disease (SCD), systemic lupus erythematosus (SLE), and solid organ transplantation (SOT).
Study design |
Blood was collected from 112 subjects at the time of H1N1/2009 vaccination and 46 ± 15 days later for hemagglutination inhibition titers and γ-interferon ELISPOT responses to H1N1/2009 vaccine and TIV; unvaccinated children also received TIV at enrollment.
Results |
A significant increase in the percentage of subjects with seroprotective hemagglutination inhibition titers to both vaccines was observed in all high-risk groups. Children with asthma and SCD were most likely to achieve seroprotective titers to H1N1/2009, whereas <50% of subjects with SOT and SLE had a seroprotective response. Subjects with SOT and SLE also had lower rates of seroprotection after TIV, and subjects with SLE had the lowest ELISPOT responses to both vaccines. Overall, 73% of healthy children exhibited protective responses to TIV; only 35% achieved seroprotection for H1N1/2009.
Conclusions |
This evaluation of immune responses to H1N1/2009 in high-risk children suggests suboptimal responses for SOT and SLE subjects, but not for subjects with SCD or asthma. Higher antigen dose, additional dose regimens, or both for immunocompromised children warrant further investigation.
Le texte complet de cet article est disponible en PDF.Mots-clés : CMI, H1N1/2009, HI, PBMC, SCD, SFU, SLE, SOT, TIV
Plan
| Supported in part by the Clinical and Translational Science Award, National Center for Research Resources, a component of the National Institutes of Health (grants UL1RR025750, KL2RR025749, and TL1RR025748), National Institutes of Health roadmap for Medical Research, and National Institutes of Health Center of Excellence for Influenza Research and Surveillance (grant HHSN266200700010C to A.F-S). Its contents are solely the responsibility of the authors and do not necessarily represent the official view of the National Center for Research Resources or National Institutes of Health. Recombinant influenza viruses for HI and ELISPOT assays were the kind gifts of John Steel and Adolfo Garcia-Sastre. The authors declare no conflicts of interest. |
Vol 160 - N° 1
P. 74-81 - janvier 2012 Retour au numéro
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