Performance of HIV-1 DNA or HIV-1 RNA Tests for Early Diagnosis of Perinatal HIV-1 Infection during Anti-Retroviral Prophylaxis - 08/12/11
, Stéphane Blanche, MD 2, 3, Carine Jasseron, MS 4, 5, Philippe Descamps, MD 1, Marie-Christine Allemon, MD 7, Nicole Ciraru-Vigneron, MD 8, Corinne Floch, MD 9, Brigitte Heller-Roussin, MD 10, Eric Lachassinne, MD 11, Fabienne Mazy, MD 9, Josiane Warszawski, MD, PhD 4, 5, 6, Christine Rouzioux, PharmD, PhD 1, 3Agence Nationale de Recherche sur le SIDA et les Hepatites virales French Perinatal Cohort
Reprint requests: Marianne Burgard, MD, Laboratoire de Virologie, Hôpital Necker, 149 rue de Sèvres, 75015 Paris, France.Abstract |
Objective |
To compare performance of testing for human immunodeficiency virus (HIV)-1 DNA and HIV-1 RNA for diagnosis of HIV-1 infection in infants receiving preventive antiretroviral therapy.
Study design |
This substudy of the French multicenter prospective cohort of neonates born to HIV-infected mothers, included 1567 infants tested for HIV with polymerase chain reaction (PCR) in a single laboratory, receiving post-natal prophylaxis, not breastfed, and having simultaneous HIV-1 DNA and RNA results before 45 days. The performance of PCR was assessed in reference to the 6-month HIV-1 RNA result.
Results |
Specificity of both HIV-1 RNA and HIV-1 DNA PCR was 100% at all ages (except 99.8% for DNA at birth); sensitivity was 58% (RNA) and 55% (DNA) at birth, and 89% at 1 month, 100% at 3 months for both, and 100% at 6 months (DNA). Concordance between HIV-1 DNA and RNA results was 0.78 and 0.81 (Kappa) at birth and 1 month and 100% at 3 and 6 months. Type of maternal and neonatal prophylaxis had no effect on sensitivity, but influenced viral load.
Conclusion |
The performances of testing for HIV-1 DNA and RNA were similar with 100% sensitivity at 3 months. At 1 month during prophylaxis, 11% of infected children had negative PCR results.
Le texte complet de cet article est disponible en PDF.Mots-clés : ART, EPF-CO1, HIV, PBMC, PCR, WHO
Plan
| Supported by Agence Nationale de Recherche sur le SIDA et les Hépatites virales, Paris, France. S.B., J.W., and C.R. serve on the Scientific Committee of the ANRS CO1 Study. The authors declare no conflicts of interest. |
Vol 160 - N° 1
P. 60 - janvier 2012 Retour au numéro
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