Expression of OmpATb is dependent on small membrane proteins in Mycobacterium bovis BCG - 08/11/11
, Anne-Béatrice Blanc-Potard a, ⁎ 
Corresponding author. Tel.: +33 4 67 14 47 26; fax: +33 4 67 14 42 86.Corresponding author. Laboratoire de Dynamique des Interactions Membranaires Normales et Pathologiques, Universités de Montpellier II et I, CNRS, UMR 5235, case 107, Place Eugène Bataillon, 34095 Montpellier Cedex 05, France. Tel.: +33 4 67 14 33 81; fax: +33 4 67 14 42 86.Summary |
Small membrane proteins emerge as a novel class of regulatory molecules in bacteria. Experiments carried out in Mycobacterium bovis BCG indicate that the ompATb gene (Rv0899), encoding a major outer membrane protein, is organized in operon with Rv0900 and Rv0901, encoding two small proteins with a predicted transmembrane domain. Fractioning experiment confirmed the association of Rv0901 with the membrane fraction. To investigate the role of Rv0900 and Rv0901 in M. bovis BCG, we have constructed a strain deleted for the whole operon as well as complemented strains carrying a deletion of Rv0900 or a frameshift mutation in either Rv0900 or Rv0901. Importantly, mutations in Rv0900 and/or Rv0901 strongly altered OmpATb expression, demonstrating that Rv0900 and Rv0901 play a regulatory role, which appears to occur at a post-transcriptional level.
Le texte complet de cet article est disponible en PDF.Keywords : Membrane protein, Mycobacterium, OmpATb, Operon, Regulation
Plan
Vol 91 - N° 6
P. 544-548 - novembre 2011 Retour au numéro
Article précédent
- Base excision and nucleotide excision repair pathways in mycobacteria
- Krishna Kurthkoti, Umesh Varshney
- The Mycobacterium tuberculosis β-oxidation genes echA5 and fadB3 are dispensable for growth in vitro and in vivo
- Kerstin J. Williams, Helena I. Boshoff, Nitya Krishnan, Jacqueline Gonzales, Dirk Schnappinger, Brian D. Robertson
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?