IgE+ cells in the peripheral blood of atopic, nonatopic, and bee venom–hypersensitive individuals exhibit the phenotype of highly differentiated B cells - 12/09/11
Abstract |
We have analyzed IgE+ cells in peripheral blood of atopic donors, donors hypersensitive to bee venom, and nonatopic control donors with two- and three-color flow cytometry. Although the percentage of IgE + cells varied among these groups, the overall phenotypic patterns were similar. Most IgE+ cells do not display typical B-cell markers, such as CD19, CD20, and CD21. A significant proportion of these cells stain for CD38, indicating that they are more differentiated. IgE+ cells express FcγRII and CD45RO, an isoform associated with an advanced level of differentiation. The majority of IgE+ cells do not coexpress other surface immunoglobulin isotypes. In the case of bee venom–hypersensitive donors, we have been able to identify a small population of IgE+ cells with a specificity for phospholipase A2, a major immunogenic component of bee venom. The phospholipase A2 + cells display a phenotype similar to that of the IgE+ cells. (J ALLERGY C LIN IMMUNOL 1995;95:587-96.)
Le texte complet de cet article est disponible en PDF.Keywords : IgE cell phenotype, enumeration, bee venom hypersensitivity, desensitization, immunotherapy mechanism
Abbreviations : DDAF, EBV, FITC, IL, PBLs, PE, PLA-2
Plan
 | From athe Department of Clinical Immunology and the bDepartment of Haematology, Flinders Medical Centre and Flinders University, Bedford Park, South Australia. |
| Supported by project funding from the National Health and Medical Research Council of Australia. |
 | Reprint requests: Professor Heddy Zola, PhD, Department of Clinical Immunology, Flinders Medical Centre, Bedford Park, South Australia 5042. |
| 0091-6749/95 $3.00 + 0 1/1/59432 |
Vol 95 - N° 2
P. 587-596 - février 1995 Retour au numéro
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