Role of LFA-1/ICAM-1-dependent cell adhesion in CD40-mediated inhibition of anti-IgM antibody-induced B-cell death - 12/09/11
Abstract |
Cross-linking of surface IgM by anti-IgM antibody caused activation-induced cell death of a surface IgM+, IgD+ human B lymphoma cell line, B104. The dying B104 cells did not show the morphology of apoptosis but did show that of necrosis. However, anti-IgM antibody caused apoptosis of another surface IgM+, IgD+ human B lymphoma cell line, DND-39. The influx of extracellular Ca2+ was necessary for the cell deaths of B104 and DND-39 caused by anti-IgM antibody. Their cell deaths were inhibited by cyclosporine. The anti-IgM antibody-induced cell death of DND-39, but not that of B104, was prevented by costimulation with anti-CD40 antibody. In human peripheral blood B-cells, anti-IgM antibody inhibited cell cycle transition induced by Staphylococcus aureus Cowan I at the G2/M interphase without inhibition of DNA synthesis. In this system, too, anti-CD40 antibody canceled the inhibitory signal transduced through surface IgM and increased the number of M phase cells. Blocking antibodies against the leukocyte function-associated antigen-1/intercellular adhesion molecule-1 system decreased the rescue effect of anti-CD40 antibody in both DND-39 cells and peripheral B-cells, which shows that leukocyte function-associated antigen-1/intercellular adhesion molecule-1-dependent cell adhesion plays an important role in the CD40-mediated inhibition of surface IgM-mediated negative signals. (J ALLERGY CLIN IMMUNOL 1995;96:1136-44.)
Le texte complet de cet article est disponible en PDF.Keywords : B-cell apoptosis, surface IgM, CD40, leukocyte function-associated antigen-1, intercellular adhesion molecule-1
Abbreviations : FITC, ICAM-1, IFN, LFA-1, PBL-B, SAC
Plan
From the Department of Pediatrics, Kyoto University Hospital, Sakyo-ku, Kyoto, Japan. |
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Supported by grants from the Ministry of Health and Welfare of Japan and from Sandoz Ltd. |
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Reprint requests: Mitsufumi Mayumi, PhD, Department of Pediatrics, Kyoto University Hospital, Shogoin-Kawaharacho 54, Sakyo-ku, Kyoto 606-01, Japan. |
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1/0/68340 |
Vol 96 - N° 6S
P. 1136-1144 - décembre 1995 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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