Prenatal analysis of rhesus CcDEe blood groups by heteroduplex generator - 10/09/11
Abstract |
OBJECTIVE: Our purpose was to determine the efficacy of the heteroduplex generator in the prenatal analysis of rhesus CcDEe blood groups.
STUDY DESIGN: A cross-sectional study was performed evaluating fetal samples from 85 women undergoing prenatal diagnosis and comparing the results with standard immunologic serotyping on cord blood delivery.
RESULTS: Of the 85 samples, 64 were tested for all CcDEe alleles; one case was discrepant. Twenty-one cases were tested solely for the D antigen. Two novel genotypes were detected in the population by heteroduplex generator and confirmed by deoxyribonucleic acid sequencing. Five cases were indeterminate because of an indistinct banding pattern.
CONCLUSIONS: Heteroduplex analysis can identify rhesus blood group alleles and is inexpensive, rapid, and does not use radioactive isotopes. (Am J Obstet Gynecol 1997;176:1084-9.)
Le texte complet de cet article est disponible en PDF.Keywords : Heteroduplex, rhesus blood groups, prenatal diagnosis
Plan
 | From the Divisions of Reproductive Genetics and Maternal Fetal Medicine, Department of Obstetrics and Gynecology,athe Department of Pathology and Laboratory Medicine,bUniversity of Pennsylvania Medical Center, The Medical Center at Princeton,cand Avitech Diagnostics, Inc.d |
| Reprint requests: Nancy C. Rose, MD, Department of Obstetrics and Gynecology, 3400 Spruce St., 106 Dulles, Philadelphia, PA 19104. |
 | 0001-9378/97 $5.00 + 0 6/1/80660 |
Vol 176 - N° 5
P. 1084-1089 - mai 1997 Retour au numéro
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