Increases in bcl-2 protein in cerebrospinal fluid and evidence for programmed cell death in infants and children after severe traumatic brain injury - 05/09/11
Abstract |
Objectives: To determine whether bcl-2, a protein that inhibits apoptosis, would be increased in cerebrospinal fluid (CSF) in infants and children after traumatic brain injury (TBI) and to examine the association of bcl-2 concentration with clinical variables. Study design: Bcl-2 was measured in CSF from 23 children (aged 2 months-16 years) with severe TBI and from 19 children without TBI or meningitis (control subjects) by enzyme-linked immunosorbent assay. CSF oligonucleosome concentration was also determined as a marker of DNA degradation. Brain samples from 2 patients undergoing emergent decompressive craniectomies were analyzed for bcl-2 with Western blot and for DNA fragmentation with TUNEL (terminal deoxynucleotidyl-transferase mediated biotin-dUTP nick-end labeling). Results: CSF bcl-2 concentrations were increased in patients with TBI versus control subjects (P = .01). Bcl-2 was increased in patients with TBI who survived versus those who died (P = .02). CSF oligonucleosome concentration tended to be increased after TBI (P = .07) and was not associated with bcl-2. Brain tissue samples showed an increase in bcl-2 in patients with TBI versus adult brain bank control samples and evidence of DNA fragmentation within cells with apoptotic morphology. Conclusions: Bcl-2 may participate in the regulation of cell death after TBI in infants and children. The increase in bcl-2 seen in patients who survived is consistent with a protective role for this anti-apoptotic protein after TBI. (J Pediatr 2000;137:197-204)
Le texte complet de cet article est disponible en PDF.Abbreviations : CSF, ELISA, PCD, TBI, TUNEL
Plan
Supported by grants from the Children's Hospital of Pittsburgh Research Advisory Committee (R.S.B.C.); National Institutes of Health Child Health Research Center 5P30 HD28836 (R.S.B.C.); National Institute of Neurologic Diseases and Stroke KO8 NS01946 (R.S.B.C.), RO1 NS38620 (R.S.B.C., M.C.), 2P50 NS30318-04A1 (P.M.K., S.R.W., S.H.G.), and RO1 NS01809 (P.D.A.); and the Center for Disease Control/University of Pittsburgh Center for Injury Research and Control (P.M.K., K.J.). |
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Reprint requests: Robert S. B. Clark, MD, Division of Pediatric Critical Care, Children's Hospital of Pittsburgh, 3705 Fifth Ave, Pittsburgh, PA 15213. |
Vol 137 - N° 2
P. 197-204 - août 2000 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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