Eotaxin induces a sustained reduction in the functional adhesive state of very late antigen 4 for the connecting segment 1 region of fibronectin - 04/09/11
Abstract |
Background: Eosinophils that have bound to extracellular matrix proteins, such as the connecting segment 1 (CS-1) region of fibronectin, need to deadhere before undergoing chemotaxis through the extracellular matrix. Objective: We have investigated whether eotaxin can regulate the strength of eosinophil adhesion to the CS-1 region of fibronectin. Methods: We have used a micropipette single-cell adhesion assay to determine the force of eosinophil adhesion to the CS-1 region of fibronectin. Results: Eosinophils bound to CS-1 with high avidity, and this binding could be inhibited with neutralizing antibodies to ⍺4 integrins expressed by eosinophils or with neutralizing antibodies to CS-1. Eosinophils incubated in the presence of eotaxin demonstrated a transient increase in the force of eosinophil adhesion to CS-1, which was followed by a more sustained reduction in the force of eosinophil adhesion to CS-1, as assessed in the micropipette single-cell adhesion assay. This decreased binding of eosinophils to CS-1 was not due to alterations in very late antigen 4 (VLA-4) receptor number, as assessed with FACS analysis, or alterations in VLA-4 receptor distribution, as assessed with immunofluorescence microscopy. Conclusions: These studies suggest that eotaxin can cause a transient increase followed by a more sustained reduction in the functional force of VLA-4 adhesion to CS-1 and thus promote deadhesion of CS-1 adherent eosinophils in the extracellular matrix. (J Allergy Clin Immunol 2000;106:933-40.)
Le texte complet de cet article est disponible en PDF.Keywords : Eosinophil, adhesion, integrin, eotaxin
Abbreviations : CS-1:, GTP:, MAPK:, MCP-3:, VLA-4:
Plan
Supported by National Institutes of Health (NIH) grants AI33977 and AI38425 (D.H.B.), as well as by the UCSD General Clinical Research Center NIH grant MO1 RR00827. |
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Reprint requests: David Broide, MBChB, University of California San Diego, 9500 Gilman Dr, (MC 0635), La Jolla, CA 92093-0635. |
Vol 106 - N° 5
P. 933-940 - novembre 2000 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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