Kinetics of IL-10 production after segmental antigen challenge of atopic asthmatic subjects - 04/09/11
Abstract |
Background: IL-10 is an anti-inflammatory cytokine made by lymphocytes, monocytes-macrophages, and eosinophils, and it may have an important role in regulating the asthmatic inflammatory response. IL-10 levels have been reported to be reduced in asthmatic airways, potentially contributing to more intense inflammation. Objective: We sought to determine whether IL-10 levels were deficient in patients with mild asthma compared with controls and to determine whether IL-10 levels were associated with the resolution of eosinophilic inflammation. Methods: We quantified IL-10 levels in the bronchoalveolar lavage (BAL) fluid (ELISA), BAL cells (quantitative immunocytochemistry), purified alveolar macrophages-monocytes studied ex vivo (ELISA), before (day 1) and after (24 hours [day 2], 1 week [day 9], and 2 weeks [day 16]) segmental antigen challenge (SAC), and investigated the effect of glucocorticoid treatment on ex vivo macrophage-monocyte IL-10 production. Results: IL-10 levels were significantly higher in the BAL fluid of mild asthmatic subjects who demonstrated a dual reaction (both early and late) after whole lung ragweed inhalation challenge compared with nonallergic, nonasthmatic control subjects before and 24 hours and 1 week after SAC. Macro-phages-monocytes obtained before and after SAC from asthmatic patients also secreted increased amounts of IL-10 ex vivo than those from controls. Dexamethasone did not significantly change spontaneous IL-10 secretion from macrophages-monocytes in vitro. Quantitative immunocytochemical analysis of BAL cells demonstrated increased IL-10 in macrophages 24 hours after SAC and a similar trend in eosinophils. Conclusion: IL-10 is not deficient in mild asthma. Furthermore, BAL IL-10 levels are significantly higher in asthmatic subjects with a dual response than in control subjects before and after SAC. The increase in IL-10 was coincident with the initial increase in BAL eosinophils, although BAL eosinophilia persisted after IL-10 levels had returned to baseline, suggesting that the increased IL-10 levels could not promptly terminate this localized eosinophilic response. (J Allergy Clin Immunol 2000;106:880-6.)
Le texte complet de cet article est disponible en PDF.Keywords : Interleukin-10, allergic asthma, cytokines, macrophages, eosinophils, glucocorticoids, segmental antigen challenge, bronchoalveolar lavage, immunocytochemistry
Abbreviations : AAD (or AD), AAS (or AAS), BAL, RWAU, SAC
Plan
 | Supported in part by grant No. AI24509 from the National Institutes of Health, National Institute of Allergy and Infectious Diseases, Bethesda, Md. R. M. P. is a recipient of a Pulmonary Fellowship Award from Glaxo-Wellcome. J. G. Z. is the recipient of Mentored Clinical Scientist Development Award No. HL03663 from the National Institutes of Health, National Heart, Lung, and Blood Institute, Bethesda, Md. |
| Reprint requests: Stephen P. Peters, MD, PhD, Jefferson Medical College, 805 College Building, 1025 Walnut St, Philadelphia, PA 19107-5083. |
Vol 106 - N° 5
P. 880-886 - novembre 2000 Retour au numéro
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