Deletion-targeted multiplex PCR (DTM-PCR) for identification of Beijing/W genotypes of Mycobacterium tuberculosis - 01/09/11

Doi : 10.1016/j.tube.2007.05.014

Jing Chen ^a,^b,^c, Anthony G. Tsolaki ^f, Xin Shen ^d, Xi Jiang ^e, Jian Mei ^d, Qian Gao ^a,^b,^c,^⁎
^a Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, 138 Yi Xue Yuan Road, Shanghai 200032, China
^b Institute of Medical Microbiology, Fudan University, Shanghai 200032, China
^c Institute of Biomedical Sciences, Fudan University, 138 Yi Xue Yuan Road, Shanghai 200032, China
^d Department of TB Control, Shanghai Municipal Centers for Disease Control and Prevention, Shanghai 200336, China
^e State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 220 Handan Road, Shanghai 200433, China
^f Biosciences, School of Health Sciences and Social Care, Brunel University, Uxbridge, London UB8 3PH, UK

Corresponding author. Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, 138 Yi Xue Yuan Road, Shanghai 200032, China. Tel.: +862154237195; fax: +862154237971.

Summary

Beijing/W strains of Mycobacterium tuberculosis cause the vast majority of tuberculosis cases in Shanghai, China. Such highly prevalent strains are considered as hypervirulent and are often associated with multi-drug resistance, treatment failure and HIV status. We present a reliable and fast detection method to identify these Beijing/W strains, which can be applied to screening large numbers of samples at low cost. Using this Deletion-Targeted Multiplex PCR (DTM-PCR) method for detecting these strains, we obtained 100% sensitivity and specificity.

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Keywords : Mycobacterium tuberculosis, Beijing/W genotype, DTM-PCR

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Vol 87 - N° 5

P. 446-449 - septembre 2007 Retour au numéro

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Deletion-targeted multiplex PCR (DTM-PCR) for identification of Beijing/W genotypes of Mycobacterium tuberculosis - 01/09/11

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