Increased expression of alpha-1-antitrypsin, glutathione S-transferase ? and vascular endothelial growth factor in human pancreatic adenocarcinoma - 01/09/11

Doi : 10.1016/S0002-9610(02)01105-4

Aaron L Trachte, M.D. ^a,^b, Sara E Suthers, M.D. ^a,^b, Megan R Lerner, H.T., A.S.C.P. ^a,^b, Jay S Hanas, Ph.D. ^a,^d, Eldon R Jupe, Ph.D. ^a,^c, Anna E Sienko, M.D. ^e, Adekunle M Adesina, M.D. ^e, Stan A Lightfoot, M.D. ^e, Daniel J Brackett, A.S. ^a,^b, Russell G Postier, M.D. ^a,^b,^⁎
^a Department of Surgery, University of Oklahoma Health Sciences Center, P.O. Box 26901, Oklahoma City, OK 73104, USA
^b Veterans Administration Medical Center, Oklahoma City, OK, USA
^c Immunology and Cancer, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA
^d Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA
^e Department of Pathology, University of Oklahoma Health Sciences Center and Veterans Administration Medical Center, Oklahoma City, OK, USA

^*Corresponding author. Tel.: +1-405-271-7912; fax: +1-405-271-3919.

Abstract

Background

This study was designed to investigate abnormalities in gene expression in ductal adenocarcinoma of the pancreas using cDNA arrays.

Methods

Gene expression in pancreatic ductal adenocarcinoma was compared with normal pancreatic tissue controls. Specimens from 5 patients with pancreatic adenocarcinoma were taken fresh at operation and analyzed using commercially prepared cDNA arrays evaluating approximately 2,000 genes. Immunohistochemical staining was used to confirm protein expression of selected genes.

Results

Alpha-1-antitrypsin (A1AT) and glutathione S-transferase π (GSTP) were significantly up-regulated in all 5 tumors. Vascular endothelial growth factor (VEGF) was up-regulated in 4 of the 5 patients. Immunohistochemical staining verified the overexpression of each of these genes.

Conclusions

A1AT, GSTP, and VEGF are overexpressed in human pancreatic adenocarcinoma specimens taken fresh at operation. To our knowledge, this is the first study of human pancreatic ductal adenocarcinoma demonstrating the up-regulation of these genes using gene expression arrays.

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Keywords : Pancreatic adenocarcinoma, Alpha-1-antitrypsin, Glutathione S-transferase π, cDNA array, Vascular endothelial growth factor